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20 Cards in this Set
- Front
- Back
- 3rd side (hint)
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E. Coli
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BAP: Gamma or Beta
medium to large grayish colonies mothball/ammonia smell. MAC: Pink colonies, pink cloudiness in media |
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Klebsiella
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BAP: Gamma, large mucoid colonies, whitish to grey, yeast/chlorine odor
MAC: Pink Mucoid colonies |
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Pasturella
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BAP: small grey colonies resembling strep.
MAC: No Growth |
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Proteus
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BAP: swarming growth covering entire plate, turns BAP brown, burnt chocolate odor
MAC: tan colonies, limited swarming |
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Pseudomonas
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BAP: Gamma, irregular spreading colonies.
Beta: metallic sheen, fruity odor MAC: tan irregular colonies, turns clear agar green |
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Salmonella
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BAP: gamma, smooth (do not pop up off media) grey to white colonies. No odor looks like gamma staph
MAC: tan colonies |
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Oxidase test
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Obtain from lay down w swab
1-2 drops reagent Positive dark purple |
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How to perform TSI
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Select colony with wire, stab the butt, streak wire up the slant
Leave cap on loose incubate 35-37° C |
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TSI results are reported as:
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Slant/butt/gas/H2S
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Possible slant/butt results on TSI
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A: Acid
K: Alkaline N: Neutral If H2S positive butt is always acid. |
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Positive Gas Results on TSI
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Tears or bubbles in the agar
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Positive H2S Results on TSI
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Black color on stab line
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How to perform SIM
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Stab butt, leave cap on loosely, incubate 24-48 hr
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SIM results are reported as:
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H2S/Indole/Motility
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Recorded in the same order as the initials of the test
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Positive results of SIM
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H2S: Black
Indole: Red color after Indole reagent Motility: Cloudy Agar If black is contained to stab line: NOT motile, if black spreads throughout media IS motile. |
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How to perform Simmons Citrate & Positive Result
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Streak slant, do NOT stab, incubate
Positive: Blue Neg: Green |
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How to perform Phenylalanine test
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Streak the slant, do NOT stab,
Incubate 18-24 hr |
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Positive Phenylalanine test
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After adding 1-2 drops Ferric Chloride (FeCl), immediate color change to green. No green negative.
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How to perform Urea test
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Mix colony into broth or streak wire, incubate overnight.
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Possible Urea test results
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Pink: positive
Salmon: weak positive Yellow: negative |
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