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53 Cards in this Set
- Front
- Back
D-Glucose + H2O +O2 with what enzyme results in H2O2 + D-Glucose
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glucose oxidase
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spemin that may be used for glucose are
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serum, plasma,
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what are the serum requirments for glucose:
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serum must be separted from the clot promtly since the rate of glucose decrease is approximately 7% per hour
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for the glucose method what was used as a blank
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reagent
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glucose absorbance is read at
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500nm
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how is the concentration of teh unknown determined in glucose
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beers law, abs of (unk/knw)*conc of std=conc of unk
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a fasting glucose result of less than 70 is
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decreaed
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a fasting glucose result that is from 70-99 is
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normal
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a fastin gglucose that is 100-125 is
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pre diabetes (impaired fasting glucose)
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a fasting glucose result that is 126 and above is
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diabetes
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serum glucose increases are seen in
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diabetes mellitus, severe stres (temporary elevation), cerebrovascular accidents, pancreatic destruction, cushing's syndrome
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serum glucose decrease seen
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tumur of pancreatic islets cells, deficiency of glucocorticoids, severe liver disesae, non pnacreatic tumurs
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what intereferes with glucose
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hemolysis
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the final glucose absorbance is stable for
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15 minutes
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glycohemoglobin is formed by
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the adduction of glucose to the n terminal of the hemoglobin beta chian
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the glycohemoglobin reflects
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blood glucose concentration over ane xtended period of time becaues it is not reversible
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measuring glycohemoglbin is useful in determining
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how well a diabetic patient is controlling their glucose intake
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is the glycohemoglobin method meant to diagnose diabetes
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no
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a hemolyzed sample of blood is added to a cation exchange resin, non glycatd hemoglobin will bind to the resin, and glychohemoglobin will elute first
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glycohemoglobin method
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is used to spearate the suprnatant containing the glycohemoglobin from the resin
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filter
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the percent glycohemoglobin is determined by
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measuri9ng the absorbance at 415 nm of glycohemoglobin fractiopn and the total hemoglobin fraction
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specimen used for glycohemoglobin are
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whole blood with EDTA
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the formula for standard ratio in the glycohemoglobin method is
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glycohemoglobin absorbance/total hemoglobin absorbance
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the formula for unknown ratio is
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glycohemoglobin absorbance/totalhemogloban absorabnce
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the unknown percent is calculated in the glycohemoglobin method by
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taking the uknown ration dividing that by the standard ratio, and multiplying by the concentration of teh standard
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the glycohemoglobin target range is
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less than 5.7
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prediabetes results for the glycohemoglobin include
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5.7%-6.4%
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the glycohemoglobin result that confers probable diabetes mellitus
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is greater than or equal to 6.5%
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what can interfere with the glycohemoglobin method
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temparature
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which binds more tightly HbA, HbS, or HBC
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HbA
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results may be inconsistent in patients who have
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opiate addiction, lead-poisinging, uremia, alcoholism, and ingesetion of large doses of aspirin
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the glycosylated hemoglobin assay is linear to
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20%A1C
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biuret is a product of urea that is formed when
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urea is decomposed at 180C
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the alkaline copper reagent reacts with in the biuret method
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substanaces containing two or more peptide bonds, producing a blue violet color
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specimen used for biuret are
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plasma and serum, hemolysis and lipemia are avoided
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contains sodium hydroxide, potassium iodide, copper sulfate, potassium sodium tartate
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buiret
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bovine fraction V albumin
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is the standard used for biuret
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biuret uses
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beers law
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serum total protein increasesa are seen in
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hemoconcentration, dehydration, multiple myeloma, chronic infection, rheumatoid arthritis,
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serum total protein decreases in
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liver disease, malnutrition, toxemia, congestive heart vailure, severe burns and nephritis and nephrosis
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the albumin method uses what dye
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bromcresol green
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bromcresol green absorbs at
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630nm
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BCG is increaed by the presenc of albumin at a ___ pH
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acid
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the albumin method follows beers law
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yea
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reference range for albumin in serum is
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3.5-5.5 g/dL
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albumin is increaed in
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dehydration, hemoconcentration
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serum albumuin is decreaed in
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chronic liver disease, malnutrition, advanced malignancies, starvation, toxemia, congestive heart failure, nephritris, nephrosis
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the final absorbance for albumin is stable for
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2 hours
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SSA is used on what samples
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urine and spinal fluid
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reference range for protein in CSF
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is 15-45 mg/dL
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the reference range for urine in a ssa method is
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100 mg/24 hors
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csf protein increases are seen in
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bacterial meningities, latet syphilis, meningeal inflammation, brain tumor, subarachnoid hemorrhage
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urine protein increases are seen in
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multiple myeloma, macroglobulinemia, renal tubular disease, pyelonephritis, glomerulonephritis
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