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53 Cards in this Set

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D-Glucose + H2O +O2 with what enzyme results in H2O2 + D-Glucose
glucose oxidase
spemin that may be used for glucose are
serum, plasma,
what are the serum requirments for glucose:
serum must be separted from the clot promtly since the rate of glucose decrease is approximately 7% per hour
for the glucose method what was used as a blank
reagent
glucose absorbance is read at
500nm
how is the concentration of teh unknown determined in glucose
beers law, abs of (unk/knw)*conc of std=conc of unk
a fasting glucose result of less than 70 is
decreaed
a fasting glucose result that is from 70-99 is
normal
a fastin gglucose that is 100-125 is
pre diabetes (impaired fasting glucose)
a fasting glucose result that is 126 and above is
diabetes
serum glucose increases are seen in
diabetes mellitus, severe stres (temporary elevation), cerebrovascular accidents, pancreatic destruction, cushing's syndrome
serum glucose decrease seen
tumur of pancreatic islets cells, deficiency of glucocorticoids, severe liver disesae, non pnacreatic tumurs
what intereferes with glucose
hemolysis
the final glucose absorbance is stable for
15 minutes
glycohemoglobin is formed by
the adduction of glucose to the n terminal of the hemoglobin beta chian
the glycohemoglobin reflects
blood glucose concentration over ane xtended period of time becaues it is not reversible
measuring glycohemoglbin is useful in determining
how well a diabetic patient is controlling their glucose intake
is the glycohemoglobin method meant to diagnose diabetes
no
a hemolyzed sample of blood is added to a cation exchange resin, non glycatd hemoglobin will bind to the resin, and glychohemoglobin will elute first
glycohemoglobin method
is used to spearate the suprnatant containing the glycohemoglobin from the resin
filter
the percent glycohemoglobin is determined by
measuri9ng the absorbance at 415 nm of glycohemoglobin fractiopn and the total hemoglobin fraction
specimen used for glycohemoglobin are
whole blood with EDTA
the formula for standard ratio in the glycohemoglobin method is
glycohemoglobin absorbance/total hemoglobin absorbance
the formula for unknown ratio is
glycohemoglobin absorbance/totalhemogloban absorabnce
the unknown percent is calculated in the glycohemoglobin method by
taking the uknown ration dividing that by the standard ratio, and multiplying by the concentration of teh standard
the glycohemoglobin target range is
less than 5.7
prediabetes results for the glycohemoglobin include
5.7%-6.4%
the glycohemoglobin result that confers probable diabetes mellitus
is greater than or equal to 6.5%
what can interfere with the glycohemoglobin method
temparature
which binds more tightly HbA, HbS, or HBC
HbA
results may be inconsistent in patients who have
opiate addiction, lead-poisinging, uremia, alcoholism, and ingesetion of large doses of aspirin
the glycosylated hemoglobin assay is linear to
20%A1C
biuret is a product of urea that is formed when
urea is decomposed at 180C
the alkaline copper reagent reacts with in the biuret method
substanaces containing two or more peptide bonds, producing a blue violet color
specimen used for biuret are
plasma and serum, hemolysis and lipemia are avoided
contains sodium hydroxide, potassium iodide, copper sulfate, potassium sodium tartate
buiret
bovine fraction V albumin
is the standard used for biuret
biuret uses
beers law
serum total protein increasesa are seen in
hemoconcentration, dehydration, multiple myeloma, chronic infection, rheumatoid arthritis,
serum total protein decreases in
liver disease, malnutrition, toxemia, congestive heart vailure, severe burns and nephritis and nephrosis
the albumin method uses what dye
bromcresol green
bromcresol green absorbs at
630nm
BCG is increaed by the presenc of albumin at a ___ pH
acid
the albumin method follows beers law
yea
reference range for albumin in serum is
3.5-5.5 g/dL
albumin is increaed in
dehydration, hemoconcentration
serum albumuin is decreaed in
chronic liver disease, malnutrition, advanced malignancies, starvation, toxemia, congestive heart failure, nephritris, nephrosis
the final absorbance for albumin is stable for
2 hours
SSA is used on what samples
urine and spinal fluid
reference range for protein in CSF
is 15-45 mg/dL
the reference range for urine in a ssa method is
100 mg/24 hors
csf protein increases are seen in
bacterial meningities, latet syphilis, meningeal inflammation, brain tumor, subarachnoid hemorrhage
urine protein increases are seen in
multiple myeloma, macroglobulinemia, renal tubular disease, pyelonephritis, glomerulonephritis