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10 Cards in this Set
- Front
- Back
Give the Results for Salmonella:
-MaConkey -XLD Agar -Hektoen -TSL |
**Salmonella**
No lactose fermentation, Alkaline slant,black deep, **H2S PRODUCER** -MaConkey- CLEAR--> no lactose fermentation -XLD Agar- BLACK --> H2S --> FeS -Hektoen- BLACK --> H2S, no carbon fermentation -TSL- RED over BLACK (Red=glucose fermenter, Black=H2S) |
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Give the results for Shigella:
-MaConkey -XLD Agar -Hektoen -TSL |
***Shigella***
Non-lactose fermentation is classic. Alkaline slant, acid deep with No H2S -MaConkey- CLEAR --> NO LACTOSE FERMENTATION -XLD Agar- CLEAR-->no xylose, sucrose or lactose fermentation -Hektoen- CLEAR--> no sucrose, lactose or salicin fermentation -TSL- RED over YELLOW (Red=only uses glucose, YELLOW=no H2S) |
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Give the results for 0157 E.coli:
-MaConkey Sorbitol -MaConkey -XLD Agar -Hektoen -TSL |
***0157 E.Coli***
SORBITOL NEGATIVE, xylose and LACTOSE FERMENTING. Acid slant, acid deep, H2S negative -MaConkey Sorbitol- CLEAR--> SORBITOL (-) -MaConkey- PINK-->LACTOSE FERMENTER -XLD Agar- YELLOW-->xylose, sucrose or lactose fermentation -Hektoen- YELLOW-->sucrose, lactose or salicin fermentation -TSL- YELLOW over YELLOW (ferments all sugars, no H2S) |
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This organism often contaminates beef. Most cases involve undercooked hamburgers
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0157 E.coli
Patient will be nauseated but NOT VOMITING. Watery diarrhea turns to bloody diarrhea |
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Why are antibiotics contraindicated in 0157 E.coli?
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ABX are thought to lyse the organism and release shiga toxin into pt.
Supportive treatment is recommended |
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Pathogenesis:
Invasion of M cells by means of ACTIN Stool will have mucous and blood streaks Most often spread fecal-orally |
Shigella
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-Can be picked up by WBCs and transferred to lymph nodes where it can enter blood and eventually seed in gall bladder
-Often contaminates chicken or other food products |
Salmonella
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What is a Kirby Bauer Susceptibility study?
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Uses diameter of zone of inhibition to determine if drug is sensitive of resistant
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What is Minimal Inhibitory Concentration (MIC)?
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Measures the lowest concentration of antibiotic that inhibits the growth of test organisms
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What is the relationship b/t Kirby Bauer Susceptibility and Minimal Inhibitory Concentration studies?
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INVERSE RELATIONSHIP between Size of Kirby Bauer inhibition zone and MIC
(Larger zone = smaller MIC) |