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14 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Chi square Analysis. Explain & formula |
When genotype of organisms is known, modes of inheritance allows us to predict the types of gametes that will be produced. Genetic cross. Chi square test measures How much the observed number deviates from the expected. X^2 = €[(o-e)^2/e] . Data sets that chi-squat value exceeds 0.05 level of significance deviate from the texst hypothesis |
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DNA extraction |
Precipitation with alcohol. Because in the presence of salt DNA & RNA precipitate from solutions containing ethanol or isopropanol. DNA unspoiled and sticks to spooling rod. Spooled/Purified DNA can then be loaded onto agarose gel. |
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DNA seperate during electrophoresis by |
Size and charge. |
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Why is leading dye used? |
So that DNA bands are visible on the gel. That way one can determine the distance traveled. Slight negative charge. Adds weight so that sample does not float put of the wells on the gel. . |
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What UV sensitive gel is used to interpret and see dna gel results? |
Ethidium Bromide |
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DNA restriction digestion of Lambda DNA lab |
DNA splicing is the cutting and linking of DNA. Tool in biotech. Remove a functional DNA fragment or gene from one organism and combine our with DNA of another top seed how the gene works. |
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What is a restriction enzyme? |
*Used to cut out target gene from the rest of the chromosome. *proteins that cut at specific sites. There are thousands of specific restriction enzymes |
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EcoRI recognition site |
5' GAATTC 3' (Give All Animals To The Circus) I________ I ...has 3 restriction sites in lambda DNA. |
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PstI recognition site |
5' CTGCAG 3' (Circus Tigers Growl Cougar Also Growl) Backwards cut ___________I I 6 sites on lambda dna |
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HindIII recognition site |
5' AAGCTT 3' (All American Girls Can Tip Toe) I______ I. Cut |
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PCR 'crime scene investigation' |
Looking for special/unique polymorphism sequences. Look for STR : short tandem repeats RFLP: restriction fragment length polymorphism. PCR: produces large amounts of specific pieces of DNA from trace amounts. Requirements for PCR is 1 single DNA template stand, 2 DNA primers, and 4 nucleotide blocking subunits of DNA A,G,T,C, and a buffer. Denature: 94*C Annealing: 52*C Extend: 72*C |
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p53 Li-Fraumeni syndrome |
p53 is a tumor suppressor gene. In lab mutant sequence at hot spot (central region of gene ) mutations detected in high fRequencies. Codon 175&165 in exon 5. 196&213 in exon 6, other in exon 7&8. |
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Huntington's disease lab |
*Mode of inheritance is dominant. *trinucleotide repeat: CAG * 42-100 repeats for diagnosis *restriction enzyme: SstI 5' GAGCT I C 3' ---------I 3'C I TCGAG 5' *size of usage sequence if normal is 1kb (Girls And Guys Can Take Cock)
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Sickle Cell |
*Genetic mutation: A to T change in the 6th codon of b-globin. *restRiction enzyme: MstII RecognItion sequence: 5' CCTGAGG 3' * 1.7kb uncut *1.4kb if mutation present *1.2kb mutation NoT present. |
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