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21 Cards in this Set
- Front
- Back
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what are the two terminuses of the amino a caleed
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amino N
carboxyl C |
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what are the four structures of proteins
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aa seq
alpha beta 3D multiple protein interactions |
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what is always at the 3 prime end that binds the aa
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CCA
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how many kinds of tRNA are there
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20. one per aa
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what are svedburg units based on
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mass and conformation
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what makes up the ribosome
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rRNA and ribosomal protein
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where is the ribosome assembled
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in the nucleolus
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what are the three sites on the ribosome
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EPA
exit peptidyl where peptides are made acceptor where all charged tRNAs enter except initiating met |
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what is the sine delgarno seq
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in prok. the seq in mRNA that is complementary to the 16s small subunit rRNA where the ribosome will line up near the AUG
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how is initiation in euk different
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cap and poly A tail interact with the ribosome and then the ribosome tracks down the mRNA until it finds the first AUG
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what is the SD seq rich in
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AUG
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in addition to the ribosome subunits, the mRNA and fmet tRNA there are numerous ______ ______, more in euk than prok
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initiation factors
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t or f. initiators always help with binding
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false. some prevent binding
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name the translation components
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small subunit
large subunit initiation factors GTP elongation factors many triplet codons mRNA tRNA |
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describe the assembly of the ribosomes in prok
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mRNA binds to small subunit along with initiation factor
initiator tRNA fmet binds to mRNA in the Psite the large subunit binds. Initiating factors are released the next tRNA enters the A site |
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what helps with termination
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gtp dependent releasing factors
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what experiment disproves one gene one enzyme
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condidia wild type on minimal media
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describe the condidia experiment
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x ray
minimal rich |
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what is minimal media? rich?
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C and sugar only
includes aa, N bases, etc |
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where does the experiment go now
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what part of the rich media? answer: aa
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so why is one enzyme one gene wrong
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multiple precursors
subuits not all prot are enzymes |
