Genetics Chapter 13 Transposable Elements

Front 1

transposable elements were first seen in gen stud of?

Back 1

maiz

Front 2

transpos elem can move to new posit in ?

Back 2

same and diff chrom

Front 3

transpos elem are ________ percent of the human genome

Back 3

50%

Front 4

plants and animal use _______________ mech to regul tranpos

Back 4

epigenetic

Front 5

what are 2 classes of euk transp?

Back 5

class I dna---rna---dna
class II DNA--DNA

Front 6

class I elem are also called?

Back 6

RNA elements

Front 7

Do humans have reverse transcriptase?? I thought they didnt?

Back 7

I have to ask the teacher

Front 8

what are the different names for class I transp?

Back 8

1. RNA elements
2. retro elements

Front 9

what is the movement called of class I ?

Back 9

retrotransposition

Front 10

class I elements __________ be excised from the genome

Back 10

cannot

Front 11

class II elem are called DNA elements bec?

Back 11

class II elem movies itself from one site in genome to another

Front 12

class II _____________ excise from donor site

Back 12

can

Front 13

in maiz, Class ___________- led to unusual spotted kernels bec of their excision from g resp for pigm prod

Back 13

II

Front 14

Barbara mcclintock saw what occuring?

Back 14

maiz
chromos 9. would break due to 2 factors. Ds dissasociat and Ac activator

Front 15

maiz
chromos 9 breaks at _________ locus

Back 15

Ds

Front 16

maiz
breakage results in the loss of what wild type alleles?

Back 16

C,Sh, Wx

Front 17

the spotted kernel is an exampl of ?

Back 17

an unstable phenotype

Front 18

maiz
C g with Ds inserted is a

Back 18

mutant

Front 19

if Ds jumps away from C g what will happen?

Back 19

revert to normal gene

Front 20

the colorless, shrunken, not shiny part of the corn kernel was produced when?

Back 20

a chrom frag cont C,SH, Wx is lost thr break at Ds locus. then these recessive alleles on homol chrom are expressed

Front 21

maiz
new unstable alleles are prod when?

Back 21

Ds inserts in the C g. Ac can excise Ds from C g which allows the color to be express again

Front 22

c-m-Ds means

Back 22

c-mutable (DS)

Front 23

maiz
a strain with c-m(DS) and no Ac has what color kernels?

Back 23

colorless. bec Ds cant move without Ac

Front 24

maiz
a strain with c-m(DS) and Ac has what color kernels?

Back 24

spotted. bec Ac activ Ds, in some c to leave

Front 25

Ac activ Ds in some c to leave or to ?

Back 25

excise, transpose

Front 26

T or F
c-m (Ac)is always unstable

Back 26

T

Front 27

T or F
c-m (Ds) is always unstable

Back 27

F
only when Ac is also present

Front 28

Ds is probably a _______________ of Ac

Back 28

incomplete, mutated version

Front 29

autonomous elements are?

Back 29

elements that req no oth elem for their mobility

Front 30

non autonomous elem?

Back 30

req oth elem for their mobility

Front 31

Ac is a
a. auton elem
b. non auton elem

Back 31

Ac is a
auton elem

Front 32

element family

Back 32

1. auton elem
2. nonauton elem that it can mobilize

Front 33

what 3 things can a transpo do?

Back 33

1. inactiv a g
2. cause chromosom break
3. transp to new locat

Front 34

inser seq element are in?

Back 34

bact

Front 35

IS

Back 35

insertion sequence elements

Front 36

IS elements were first found in E coli in what g?

Back 36

gal operon

Front 37

phage lamda inserts next to?

Back 37

gal operon

Front 38

lambdadgal phage has?

Back 38

picked up the gal region from the bact

Front 39

phage lambda particles with gal mutation have __________ density than lambda with out gal mutat

Back 39

higher

Front 40

why do lambda particl with gal mutat have higher density?

Back 40

bec they have the gal g plus the IS, giving it more mass for the same volume

Front 41

bact
all IS have a prot called?

Back 41

transposase

Front 42

transposase is?

Back 42

an enzyme req for mov of IS elements

Front 43

all IS begin and end w?

Back 43

short inverted repeat seq

Front 44

T or F
E Coli has lots of IS

Back 44

t

Front 45

E coli
IS regions have ____________seq

Back 45

identical

Front 46

recombination betw F fact plasmid and E coli chromosome to form Hfr strains is via?

Back 46

single crossover betw an IS elem on F and IS on bact chrom

Front 47

R factor is?

Back 47

plasmid w g for resist to antib

Front 48

Drug resist genes are located on?

Back 48

transposon

Front 49

Tn stands for

Back 49

transposon

Front 50

2 types of bact transpos are?

Back 50

1. composite transpos
2. simple transpos

Front 51

bact
composite trans is?

Back 51

collect of g inbetw 2 IS

Front 52

bact composit transp has 2 IS oriented?

Back 52

opposit direct

Front 53

Inverted repeats are in _________ transposons

Back 53

composite

Front 54

IS elements in composite transposons can not?

Back 54

transpose on their own

Front 55

the IS associated w simple transposons do not have the ___ enzyme

Back 55

transposase

Front 56

composite transposons have the transposase where?

Back 56

in their IS seq

Front 57

simple transposon have the transposase in their?

Back 57

own seq, not in their IS seq

Front 58

t or f
a transposon can jump from a plasmid to anoth plasmid

Back 58

t

Front 59

first step of transposition is?

Back 59

transposase makes a staggered cut in target site DNA

Front 60

the action of transposase is similar to?

Back 60

restric enzymes that make breaks in sugar phosphate backbone of dna

Front 61

after transposon inserts inbetw the staggered ends of the dna what happens?

Back 61

host dna repair machinery repairs the breaks

Front 62

integration of transpos makes a ?

Back 62

five base pair duplication

Front 63

the five base pair duplica at site of transpos insertion is called?

Back 63

target-site duplication

Front 64

t or f
each transpos type can have diff number of duplications

Back 64

t

Front 65

prokaryotes
2 mechanisms of transposition are?

Back 65

replicative
conservative

Front 66

results of replicat pathway for transpos is?

Back 66

1. new copy in new site
2. one copy stays at old site

Front 67

prok
conservative transposition
the transposon leaves ?

Back 67

the old site and inserts into new site

Front 68

transposition
"cut and paste " is the __________ mechanism

Back 68

conservative

Front 69

replicat transposition
transposition intermed is made up of?

Back 69

a double plasmid with donor and recip being fused togeth

Front 70

replicat transpos
format of double plasmid intermed is catalyzed by?

Back 70

Tn3 encoded transposase

Front 71

replicat transposition
donor plasmid + target plasmid ------ ?

Back 71

cointegrate

Front 72

replicat transpos
cointegrate separ back into 2 plasmids by ?

Back 72

a recombinat like event

Front 73

gerry fink
studied what gene?

Back 73

HIS4

Front 74

HIS4 is involved in ?

Back 74

pathway for synth of histidine

Front 75

gerry fink studied what organism?

Back 75

yeast

Front 76

retrovirus has a ss

Back 76

RNA

Front 77

retrovirus
RNA ---DNA by?

Back 77

reverse transcriptase

Front 78

name 2 retroviruses responsible for tumours?

Back 78

MMTV
RSV

Front 79

provirus is

Back 79

ds dna copy of retroviral genome

Front 80

retrovirus and Ty1 are both flanked by?

Back 80

long terminal repeats

Front 81

LTR are?

Back 81

long terminal repeats

Front 82

gag, pol, and env are 3 what?

Back 82

prot that take part in viral replication

Front 83

why cant Ty1 elements leave the c?

Back 83

bec they dont have anything that encodes for env

Front 84

euk
retrotransposons are

Back 84

transp elem that utilize revers transcriptase to go thr a RNA interm

Front 85

class I transpos elem are?

Back 85

use revers transcriptase to transpose thr rna intermed

Front 86

retrotransposons are also called?

Back 86

class 1 transposable elements

Front 87

retrotranspos that have long terminal repeats are called?

Back 87

LTR-retrotransposons

Front 88

copia like elements of drosophilia are?

Back 88

spontant mutations w retrotransp insertions

Front 89

only _________ have transposons that transpose thr rna intermed

Back 89

euk

Front 90

euk
class II elements are also called

Back 90

dna transposons

Front 91

euk
DNA transpos act like ___________ in bact

Back 91

IS elements

Front 92

P elements in drosophilia are?

Back 92

DNA transposons

Front 93

p elements were discov by studying ?

Back 93

hybrid dysgenesis

Front 94

drosophilia
dysgenic means

Back 94

biologically deficient

Front 95

drosophilia
hybrid progeny are products of?

Back 95

M cytotype- f from the lab
P cytotype m from the wild

Front 96

drosophilia
M cytotype are ?

Back 96

f drosoph from the lab

Front 97

P cytotype are from?

Back 97

m the wild

Front 98

A f M cytotype and m P cytotype mate? what do you get?t

Back 98

hybrid dysgenesis

Front 99

a m M cytotype and a f P cytotype mate. what do you get?

Back 99

no dysgenic offspring

Front 100

drosoph
eye color white
mutat causes by a ?

Back 100

P element
transpos element insertion

Front 101

explanation for hybrid dysgenesis?

Back 101

P strains have p elements but also have a repressor

Front 102

p elements have?

Back 102

transposase
repressor

Front 103

most laboratory strains of drosoph have no?

Back 103

p elements, and thus no p repressors

Front 104

hybrid
f M type X m P type. the male does not contribute?

Back 104

any cytoplasm which has the p repressor in it. so this lets the p element transpose.