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7 Cards in this Set
- Front
- Back
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which two restriction endonucleases at specific recognition sites to produce sticky, cohesive ends? |
-EcoRI -HindIII |
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which restriction endonuclease cut at specific recognition sites to produce blunt ends? |
-HaeIII |
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which enzyme is used to make complementary DNA (cDNA) from RNA? |
- Reverse Transcriptase PCR (RT-PCR) |
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what does anneal mean? |
- to recombine DNA in the double stranded form. |
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what components does PCR require? |
- Taq polymerase - Mg2+ buffer - primers forward and reverse - dNTPs - DNA template |
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what do primers do? |
- tell polymerase when to work |
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which enzyme catalyses the formation of RNA form DNA during transcription? |
transcriptase |
