Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
8 Cards in this Set
- Front
- Back
|
PCR |
in vitro method for rapid production of large amounts of specific DNA sequences. Suitable for clinical and forensic testing because only a small amount of template DNA is required as starting material. |
|
|
Gel Electrophoresis |
separates nucleic acids based on size |
|
|
Southern blot |
complementary ssDNA probe used that is tagged w/ radioactive isotope. presence or absence points to mutation. |
|
|
RFLP |
Restriction fragment length polymorphism (polymorphism is typically defined as a sequence variation that is found in more than 1% of the population and is clinically harmless.) Single base pair change in the DNA may cause creation or destruction of a restriction site |
|
|
ASO |
Probes are usually 15-21 bases long and very sensitive. Complementary to the DNA of interest. Used to detect polymorphism genetic mutations(CFTR, SC) DNA isolated from WBC is amplified, denatured, and applied to a filter, 2 rows. Complementary probe to normal gene applied to row 1. Complementary probe to disease gene applied to row 2. Test only useful if the mutation is known. |
|
|
Allele Specific PCR |
Allele specific PCR can be used to detect single nucleotide changes. By designing primers which hybridize to the region containing the mutation/polymorphism. Internal controls can ensure that the PCR is working properly |
|
|
Northern blot |
Similar to southern but contains isolate mRNA |
|
|
Western blot |
western blot analysis can be used to detect and quantify the amount of a particular protein: electrophoresis of protein Transfer to nylon membrane. Detection using labeled antibody system (typically an enzyme label and a chemiluminescent substrate) |
