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19 Cards in this Set

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Recombinant DNA
(see notes sheet)
how to make it:
bacterium-take out plasmid-insert isolated DNA from human-put the plasmid back in the human-multiply=gene of interest

WHY USEFUL??
-produces medicine
-diagnose diseases
-vaccines (maybe edible)
Restriction enzymes
used to cut genes in the exact same spot in diff. organisms
Gel electrophoresis
used in :
-crime
-paternity
-disease/mutations
smallest fragments run fartherest down
DNA has a negative charge
DNA fingerprinting
when you identify someone by anylyzing thier DNA
Transgenic animals
an animal that carries a foreign gene that has been deliberately inserted into its genome. The foreign gene is constructed using recombinant DNA.
Genetically modified foods
(rice) very controversial
Cloning (SCNT)
reproductive/theraputic
clone=member of a population of genetically identical cells produced by a single cell
**not necessarily transgenic(containing genes from other organisms)
(see notes for diagram)
Therapeutic/ Reproductive
reproductive-entire organism
theraputic-specialized cells
Stem cell therapy
stem cells are unspecifized cells that can be made to any job in the body, they can be used to create needed body substances. controversial--are the cells living? is it right to alter them?
Gene therapy
(replace certain cells)
-manipulate DNA in order to treat individuals with genetic disorders

1. genetically engineer a virus w/ the wanted DNA
2. expose mutated cells to "good virus
3. inject cells back into human

controversy: cna it be controled? cause cancer? who has access??
PCR
amplify DNA
Antigen
a substance that prompts the production of antibodies
Antibody
used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses
Specific Defense
-distinguishes between microbes

*lymphosite (WBC)
*build up army
Non Specific Defense
when a white blood cell(defense) does not distinguish between microbes
Inflammatory Response
the second line of defense: pain, swelling, dialation of blood vessels. white blood cells destroy+clean
Pathogens
any disease-producing agent
Vaccine
a biological preparation that improves immunity to a particular disease
Self vs. non self markers
self markers let the white blood cells know that the cells are of the body, and shouldn't be attacked, visa versa