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25 Cards in this Set
- Front
- Back
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Biological catalysts that regulate the rate of chemical reactions.
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Enzymes.
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The enzyme that is found in all living organisms.
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Catalase.
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The enzyme that will decompose hydrogen perixode (substrate).
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Catalase.
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Name the two products that Hydrogen peroxide (substrate) will decompose to.
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Water and Oxygen.
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2H202 + Catalase (enzyme)--> 2H2O + O2
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Balanced equation for action of catalase on hydrogen peroxide.
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Substrate = H202
Enzyme = Catalase Products = Water and Oxygen |
Components of Catalase Activity (pH & temperature).
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What was the source of Catalase in Lab 6?
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Sheep's Blood.
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What are the external factors that will effect the catalase activity?
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Temperature and pH.
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What is DIRECTLY PROPORTIONAL to the rate of reaction?
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Amount of oxygen gas produced.
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Greater rate of reaction = Greater oxygen produced.
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Directly proportional.
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How is the rate of reaction measured?
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Measuring gas pressure of oxygen produced.
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What is the optimum pH with greatest oxygen produced?
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7.
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What will stop reaction as it denatures the enzyme (protein)?
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Very high (alkaline - base) or very low (acidic) pH.
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As temperature increases, the rate of the reaction ________.
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Rate of the reaction increases.
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Rate of reaction increases because increase in temperature causes _______.
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Increased collisions between substrate and enzyme.
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Why does rate of reaction stop at 60 Celsius?
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Because heat denatures enzyme.
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What is the optimum temperature for catalase activity?
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40 Celsius.
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As substrate concentration decreases, rate of reaction ______.
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Decreases.
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Why does rate of reaction decrease as substrate decreases?
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Less and less substrate available for collisions.
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Control is water (no hydrogen peroxide). What type of control is this and why?
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Negative control; no reaction will occur.
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As enzyme concentration decreases, rate of reaction ______.
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Decreases.
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Why does rate of reaction decrease when enzyme concentration decreases?
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Less enzyme available for collisions.
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What will happen to rate of reaction if inhibitor CuSO4 is added?
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Reaction will stop.
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Why does reaction stop once inhibitor CuSO4 is added?
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Because it binds to the active site of the enzyme (catalase).
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Why is the optimum temperature 40 Celsius?
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Any temperatures below 40 Celsius slows down collisions; any temperatures above 40 Celsius will approach denaturation.
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