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10 Cards in this Set
- Front
- Back
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What are three examples of DNA aquisition by horizontal gene transfer?
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1)Conjugation
2)Transduction 3)Transformation |
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How is DNA degraded?
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It is degraded by enzymes called restriction endonucleases.
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What are two examples of restriction endonucleases? and what do they do?
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EcoRI and HinD111. they recognize other DNA and chew them up.
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What is recombination?
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The foriegn DNA is integrated into the chromosome.
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What is the fate of DNA within the cell?
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1)Degraded by endonucleases
2)Integraded into the chromosome 3)Cirularization of DNA and plasmid formation-->get lots of copies. |
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What is a transposon?
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1)Linear DNA "Elements"
2)Possess ability to move from one location to another within a genome 3)Replicative-a copy of the transposon is made during transposition 4)Insertion elements present at the ends of transposons(inverted repeat DNA sequences) 5)Some transposons may carry additional genes- such as anitbiotic resistance |
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What is the enzyme required for transposition?
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transposases
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Transposons "duplicate" the ??
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sequence of the insertion site when they transpose
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What are the impacts of transposition?
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1)Use in molecular biology
2)Mutations(Changed DNA sequences) 3)Lowered virulence/antibiotic resistance |
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You have a cell with a transposon that hopped into LacZ. Will this medium be able to grow on
a)defined medium with glucose as sole carbon souce b)Lactose as sole carbon source c)Neither d)both |
A)Defined medium with glucose as sole carbon source
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