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3 Cards in this Set
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- Back
What is it? |
-Method to separate DNA fragments by using electricity -DNA had - charge to it moves towards + pole -small pieces move faster that large pieces -loading dye= mix of acidic dyes (- ion is colored) and sucrose +does not bind to DNA, just moves with it + sucrose will make sample heave so it will sink +dyes = xylene cyanol ( blue green) moves like big DNA, bromophenol blue (purple) moves like small DNA -DNA ladder- mixture of DNA fragments with known size- ethidium bromide or gel red stains that stick to DNA and glow in UV light hind 3 |
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