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3 Cards in this Set

  • Front
  • Back

What is it?

-Method to separate DNA fragments by using electricity


-DNA had - charge to it moves towards + pole


-small pieces move faster that large pieces


-loading dye= mix of acidic dyes (- ion is colored) and sucrose


+does not bind to DNA, just moves with it


+ sucrose will make sample heave so it will sink


+dyes = xylene cyanol ( blue green) moves like big DNA, bromophenol blue (purple) moves like small DNA


-DNA ladder- mixture of DNA fragments with known size- ethidium bromide or gel red stains that stick to DNA and glow in UV light hind 3

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