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17 Cards in this Set
- Front
- Back
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what part of neuron does Nissl stain stain
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Nissl stain stains ribonucleic acids in nucleus (nucleolus), free polysomal ribosomes, and ribosomes associated with rough ER
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What can Nissl stain reveal
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Can reveal cytoarchitecture - relative size shape and pattern of cell distribution within nucleus
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How does Nissl stain work
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Neuron cell bodies have a lot of ribosomes while axons, dendrites and glial cells dont
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Name common Nissl stains
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cresyl violet and thionine
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What does hematoxyllin-eosin stain red
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eosin gives red color to acidophilic tissue components such as collagen, cytosol, musculature and erythrocytes
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What does hematoxylin-eosin stain blue
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basophillic tissue elements like acids
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What can this stain help distinguish
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H&E stains dead or dying neurons red because cytoplasm becomes more basic
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What are myelin stains useful for
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Myelin is usually white due to fatty content, staining turns it black or blue, useful for examining disruptions of fiber tracts or demyelination
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Example of myelin stains
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Weil myelin stain, Luxol fast blue stain
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What does the Golgi stain stain
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Golgi stain stains an entire neuron with all processes by impregnation with silver
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What is the limitation of Golgi stain
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Doesnt stain every neuron
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Why electron microscopes have better resolution then light microscopes
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EM uses electrons rather then light and electrons have shorter wavelength then photon, thus give better resolutiom
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If you want to see where a neuron projects to, what type of tract tracing would you use
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Anterograde tracers
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If you want to see where projections originate from, what type of trac tracing would you use
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Retrograde tracers
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Why would one use ICC - immunocytochemistry
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to localize molecules of neurotransmission
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What does ICC use to recognize specifice proteins
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antibodies from immune system
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Explain how one can visualize speciific neuronal proteins
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Protein (antigen) is injected into the animal,antibodies are made, serum with antibodies is isolated, another animal is perfused, brain is sectioned, sections are exposed to antibody solution, antibodies bind to sites that contain antigens, proteins are visualized with fluorescent tags
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