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27 Cards in this Set
- Front
- Back
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qualities of a good push smear |
- body, monolayer, feathered edge - doesn't extend to outer margin of slide on any sides |
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why cover slip smears are preferred |
- even cell distribution especially WBCs - less damaging to cells |
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artifact of slow drying cover slip smears |
crenation |
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why Dif quick is preferred |
- fast - practical - easily maintained - provides good stain quality |
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staining technique for blood smears |
5 dips, 1 second each, in each jar |
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how to ensure good quality stains |
- replace old stains frequently - never add old stain to new stain - keep lids on tight |
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what can be observed on low magnification view of blood smears |
- overall cell numbers - locate feathered edge - cell clumping - large abnormal cells - microfalaria |
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power for observing blood cell morphology |
oil |
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where blood cells are observed and counted |
monolayer |
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value for RBC size |
mean corpuscular volume (MCV) |
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Mean Corpuscular Hemoglobin measures |
weight of hemoglobin |
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hemoglobin concentration measured as |
Mean Corpuscular Hemoglobin Concentration (MCHC) |
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MCV units |
femtoliters (fl) |
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RBCx10/RBC = |
MCV |
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normal MCV |
60-70 fl |
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picogram (pg) |
unit for MCH |
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MCH formula |
HgBx10/RBC |
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normal MCH |
20 pg |
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MCHC is measured in |
g/dl or % |
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Hgbx100/PCV = |
MCHC |
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Normal MCHC |
30-36 g/dl or % |
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most accurate of all indicies |
MCHC |
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NMB stains |
acidic cell parts (nucleus of WBC) |
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purpose of RBC indicies |
classify type of anemia |
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preanalytic variables |
breed, gender, age, fasting time, patient stress level, sample handling, sample collection |
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analytical variables |
instrument errors: improper calibration, unstable reagents, lack of maintenance |
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vacutainer for blood smear samples |
purple top - EDTA |
