Experiment To See How Temp Affects The Rate Of Milk Hydrolysis

Front 1

Step 1

Back 1

Label five 50cm3 beaker with water bath temperatures.

20,30,40,50,60

Front 2

Step 2

Back 2

Mark an X on the bottom of each beaker.

Front 3

Step 3

Back 3

Put 10g of milk powder in a beaker and then fill it with distilled water up to 100cm3 . Mix

Front 4

Step 4

Back 4

Add 10cm3 of the milk to each 50cm3 beaker.

Front 5

Step 5

Back 5

Add 1cm3 of buffer solution to each beaker and stir.

Front 6

Step 6

Back 6

Put the 20 degrees c beaker in the corresponding water bath on a white tile. Leave for 5 minutes.

Front 7

Step 7

Back 7

Add 1cm3 of the enzyme into the beaker.

Front 8

Step 8

Back 8

Use a stopwatch to record the time (s) it takes until the X becomes visible.

Front 9

Step 9

Back 9

Repeat steps 6 to 8 for each beaker at the correct temperatures.