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19 Cards in this Set

  • Front
  • Back
which type of library is this?
-unabridged version of what makes you you
-all details are there
-contains every little detail
Genomic DNA library
if the ... library is constructed properly,
-each segment of genome is represented in the library
-each segment partially overlaps another genomic fragment in the library
Genomic DNA
a ... library is representative of the genes transcribed in a tissue under particular conditions (development, physiological or environmental stress). Genes are expressed at different levels.
Source is total ... from a specific tissue or developmental stage of embryogenesis
steps for making cDNA library
-... total RNA from tissue
-... the polyadenylated mRNA
-... the mRNA into ds cDNA using reverse transcriptase
-... the cDNAs into vectors for creation of library

-alternatively, you can clone a cDNA into an expression vector that contains a shine delgarno sequence and produce the protein
In a ... library, the genes transcribed at a higher frequency will be represented more often than those transcribed at a lower frequency
in a ... library, introns, nontranscribed DNA as well as exons are equally represented
ASO analysis
-synthetic oligonucleotide
-ideal for detecting ... mutation or small .../...
-useful in screening of disease where a significant number of those affected have defined mutation
-ASO synthesized to match the normal DNA sequence hybridizes to ... complementary sequence only (and vice versa)
single base
The advantage of ASO analysis over Southern blot analysis is the ability to detect ... changes
single nucleotide
-restriction fragment length ...
-alternative alleles associated with restriction fragments that differ in ...
Classes of RFLPs
-... - leads to creation or deletion of restriction site
-... (indels, tandem repeats, translocation) - leads to change in size of restriction site
single base pair substitutions
alteration of genetic materials
direct DNA sequencing is time consuming and expensive.
-its done for ... - used commonly when no one specific mutation is responsible for disease
-all sequencing is based on dideoxyribosenucleotide
the 2 most commonly employed methods of direct DNA sequencing is ...
sanger method
automated sequencing
... is based on the idea that an individual will have a distinct pattern of VNTR (variable number of tandem repeats) at different loci (locations) on the chromosome
-paternity testing and forensic science
DNA fingerprinting
what does this describe?
-exploits the ability of a mRNA molecule to hybridize to the DNA template from which it was synthesized
-determine expression levels of hundreds to thousands of genes
-computer analysis precisely determine amount of mRNA bound generating a gene expression profile
-quick and efficient
-small, solid supports
-thousands of different genes immobilized at fixed locations
-analyze expression of many genes in a single experiment
... refers to
-introduction of a new gene into an organism
-organism whose genome has been altered using recombinant DNA techniques
what does this describe?
-normal gene copy replaced with mutant copy
-determine effects of small and subtle mutations
gene replacement
what does this describe?
-complete inactivation of normal gene
-determine possible function of normal gene in whole animal
what does this describe?
-random insertion of mutant gene in genome
-useful when mutant gene overrides function of normal gene
gene addition
In the generation of transgenic mice, you introduce the altered gene into the ... cells
embryonic stem