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19 Cards in this Set
- Front
- Back
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which type of library is this?
-unabridged version of what makes you you -all details are there -contains every little detail |
Genomic DNA library
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if the ... library is constructed properly,
-each segment of genome is represented in the library -each segment partially overlaps another genomic fragment in the library |
Genomic DNA
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a ... library is representative of the genes transcribed in a tissue under particular conditions (development, physiological or environmental stress). Genes are expressed at different levels.
Source is total ... from a specific tissue or developmental stage of embryogenesis |
cDNA
RNA |
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steps for making cDNA library
-... total RNA from tissue -... the polyadenylated mRNA -... the mRNA into ds cDNA using reverse transcriptase -... the cDNAs into vectors for creation of library -alternatively, you can clone a cDNA into an expression vector that contains a shine delgarno sequence and produce the protein |
extract
isolate convert clone |
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In a ... library, the genes transcribed at a higher frequency will be represented more often than those transcribed at a lower frequency
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cDNA
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in a ... library, introns, nontranscribed DNA as well as exons are equally represented
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genomic
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ASO analysis
-synthetic oligonucleotide -ideal for detecting ... mutation or small .../... -useful in screening of disease where a significant number of those affected have defined mutation -ASO synthesized to match the normal DNA sequence hybridizes to ... complementary sequence only (and vice versa) |
single base
insertions/deletions normal |
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The advantage of ASO analysis over Southern blot analysis is the ability to detect ... changes
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single nucleotide
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RFLP
-restriction fragment length ... -alternative alleles associated with restriction fragments that differ in ... |
polymorphism
size |
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Classes of RFLPs
-... - leads to creation or deletion of restriction site -... (indels, tandem repeats, translocation) - leads to change in size of restriction site |
single base pair substitutions
alteration of genetic materials |
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direct DNA sequencing is time consuming and expensive.
-its done for ... - used commonly when no one specific mutation is responsible for disease -all sequencing is based on dideoxyribosenucleotide |
identification
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the 2 most commonly employed methods of direct DNA sequencing is ...
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sanger method
automated sequencing |
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... is based on the idea that an individual will have a distinct pattern of VNTR (variable number of tandem repeats) at different loci (locations) on the chromosome
-paternity testing and forensic science |
DNA fingerprinting
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what does this describe?
-exploits the ability of a mRNA molecule to hybridize to the DNA template from which it was synthesized -determine expression levels of hundreds to thousands of genes -computer analysis precisely determine amount of mRNA bound generating a gene expression profile -quick and efficient -small, solid supports -thousands of different genes immobilized at fixed locations -analyze expression of many genes in a single experiment |
microarrays
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... refers to
-introduction of a new gene into an organism -organism whose genome has been altered using recombinant DNA techniques |
transgenic
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what does this describe?
-normal gene copy replaced with mutant copy -determine effects of small and subtle mutations |
gene replacement
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what does this describe?
-complete inactivation of normal gene -determine possible function of normal gene in whole animal |
knockout
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what does this describe?
-random insertion of mutant gene in genome -useful when mutant gene overrides function of normal gene |
gene addition
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In the generation of transgenic mice, you introduce the altered gene into the ... cells
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embryonic stem
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