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19 Cards in this Set

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which type of library is this?
-unabridged version of what makes you you
-all details are there
-contains every little detail
Genomic DNA library
if the ... library is constructed properly,
-each segment of genome is represented in the library
-each segment partially overlaps another genomic fragment in the library
Genomic DNA
a ... library is representative of the genes transcribed in a tissue under particular conditions (development, physiological or environmental stress). Genes are expressed at different levels.
Source is total ... from a specific tissue or developmental stage of embryogenesis
cDNA
RNA
steps for making cDNA library
-... total RNA from tissue
-... the polyadenylated mRNA
-... the mRNA into ds cDNA using reverse transcriptase
-... the cDNAs into vectors for creation of library

-alternatively, you can clone a cDNA into an expression vector that contains a shine delgarno sequence and produce the protein
extract
isolate
convert
clone
In a ... library, the genes transcribed at a higher frequency will be represented more often than those transcribed at a lower frequency
cDNA
in a ... library, introns, nontranscribed DNA as well as exons are equally represented
genomic
ASO analysis
-synthetic oligonucleotide
-ideal for detecting ... mutation or small .../...
-useful in screening of disease where a significant number of those affected have defined mutation
-ASO synthesized to match the normal DNA sequence hybridizes to ... complementary sequence only (and vice versa)
single base
insertions/deletions
normal
The advantage of ASO analysis over Southern blot analysis is the ability to detect ... changes
single nucleotide
RFLP
-restriction fragment length ...
-alternative alleles associated with restriction fragments that differ in ...
polymorphism
size
Classes of RFLPs
-... - leads to creation or deletion of restriction site
-... (indels, tandem repeats, translocation) - leads to change in size of restriction site
single base pair substitutions
alteration of genetic materials
direct DNA sequencing is time consuming and expensive.
-its done for ... - used commonly when no one specific mutation is responsible for disease
-all sequencing is based on dideoxyribosenucleotide
identification
the 2 most commonly employed methods of direct DNA sequencing is ...
sanger method
automated sequencing
... is based on the idea that an individual will have a distinct pattern of VNTR (variable number of tandem repeats) at different loci (locations) on the chromosome
-paternity testing and forensic science
DNA fingerprinting
what does this describe?
-exploits the ability of a mRNA molecule to hybridize to the DNA template from which it was synthesized
-determine expression levels of hundreds to thousands of genes
-computer analysis precisely determine amount of mRNA bound generating a gene expression profile
-quick and efficient
-small, solid supports
-thousands of different genes immobilized at fixed locations
-analyze expression of many genes in a single experiment
microarrays
... refers to
-introduction of a new gene into an organism
-organism whose genome has been altered using recombinant DNA techniques
transgenic
what does this describe?
-normal gene copy replaced with mutant copy
-determine effects of small and subtle mutations
gene replacement
what does this describe?
-complete inactivation of normal gene
-determine possible function of normal gene in whole animal
knockout
what does this describe?
-random insertion of mutant gene in genome
-useful when mutant gene overrides function of normal gene
gene addition
In the generation of transgenic mice, you introduce the altered gene into the ... cells
embryonic stem