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27 Cards in this Set
- Front
- Back
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Bacteria growth
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inceases in number of cell
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Qualitative
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what organism is growing
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Quantitative
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how much bacteria is present, cell count
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Spectrophotometer
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measure turbidity of liquid sample
-amount of light absorbed or transmitted is comparable to the number of bacteria cell in solution sample. |
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>Absoption
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higher cell count, vice versa
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>Transmission
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lower cell count, vice versa
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Generation time
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time takes for bacteria population to double
-genetically determined -influenced by Nutritional and Enviroment Factor |
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*Lag phase
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bacteria cell are metabolically ACTIVE, but not started reproducing
-species specific, media dependent. |
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*Log phase
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cell are reproducing at exponential rate
-number of cell double every generation time |
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Stationary phase
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rate of cell reproduction is equal to the rate of cell dying
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Death phase
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cell dying at exponential rate
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Aerobic
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organisms require oxygen for respiration
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Obligate Aerobes
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Organism solely rely on oxygen for respiration
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MicoAerophille
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uses oxygen for respiration, but at a lower level, in atmosphere
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Anaerobic
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do not use oxygen for respiration and not able to grow in presence If oxygen is presences
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Aerotolerant anaeobes
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do no use oxygen for respiration, but can grow if oxygen is presence
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Facultative anaerobes
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can grow with or without oxygen
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Coccus
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spherical bacteria, capable of dividing in any direction
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GasPak Jar and specialized media
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different method to allow anaerobic growth
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Bacillus
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Rod shaped bacteria, divided end to end
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Spirillum
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wavy shape, rigid
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Spirochetes
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coiled or cork screw shape, flexible
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Agar plate
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measure size, 1-3 mm
-elevation, shape, edge, surface texture and pigmented |
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Catalase test
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used to differentiate gran positive Cocci morphology
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Catalase>bubble
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positive, micrococcus OR staphylococcus
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Catalase> no bubble
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negative, enterococcus OR streptococcus
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Enzyme catalase
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break down H2O2(toxic cell Hydrogen peroxide) into water and oxygen
- 2H2O2 --> H2O+O2 increses |
