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181 Cards in this Set
- Front
- Back
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Non hemolytic except
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E.coli
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Antigens
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Capsule(K)
Flagella(H) Cellwall(O) |
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Enterobacteriacieae characteristics
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fermentative, gram (-), facultative anaerobes,NS, motile exc Tatumella, oxidase(-), catalase(+)
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Most enterobac are present in intestinal tract as commensal except
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Salmonella, shigella, yersinia
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Enterobac are NE exc.
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Klebsiella, enterobacter
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NLF
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EEHMPPSSSY
Edwardsiella Erwinia Hafnia Morganella Proteus Providencia Salmonella Shigella Serratia Yersinia Colorless on macconkey, pathogenic |
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Escherichia is predominant in..
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Large colonies
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Can cause bacteremia and isolated from urinary tract
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KEP
K. pneumoniae E. Coli P.mirabilis |
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Cause diarrhea
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YESS
Yersinia E.coli Salmonella Shigella |
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Antibiotic resistant
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SEC
Serratia Enterobacter Citrobacter |
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Normal flora of GIT and can be opportunistic
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CEEKSMPP
Citrobac Enterobac Edwardsiella Klebsiella Serratia Morganella Proteus Providencia |
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Classisfication based on LACTOSE FERMENTATION
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LF
NLF |
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Lactose fermention medium used
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MacConkey agar (-)
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If LF grown on BAP...
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Large grayish colonies
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LF species
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KEE
Klebsiella Enterobacter Esscherichia PINK NON PATHOGENIC |
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Escherichia is predominant in..
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Large intestines
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Common name e.coli
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The colon baccilus
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Bacteroides fragilis
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Predominant in stool
Strictly anaerobes Betahemo on BAP |
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Most commonly isolated human pathogen causing uti, wound inf, pneumonia, meningitis, septicemia, variety of GIT diseases, INDEX FOR FOOD AND WATER INFECTION, normal flora of GIT
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Escherichia coli
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Morphology of e.coli
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Short and plump rods
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Strains of e.coli
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E.coli variety communior-ferments sucrose
Variety communis-does not ferment sucrose E.aurescens-yellow orange pigment production |
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No 1 cause of UTI
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E.col
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No 1 cause of UTI
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E.col
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No 2 cause of e.coli
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Neonatal meningitis
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Virulence factors of e.coli
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Endotoxin
Common pili K1 antigen |
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Bacterial substance that exerts its effect intestinal tract
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Enterotoxins
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Virulence factors of e.coli
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Endotoxin
Common pili K1 antigen |
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Bacterial substance that exerts its effect intestinal tract
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Enterotoxins
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Enteropathogenic e.coli (epec)
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E.coli 0111
E.coli 0114 Mucous with blood |
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Virulence factors of e.coli
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Endotoxin
Common pili K1 antigen |
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Bacterial substance that exerts its effect intestinal tract
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Enterotoxins
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Enteropathogenic e.coli (epec)
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E.coli 0111
E.coli 0114 Mucous with blood |
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Enteroinvasive e.coli
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E.coli 0124
E.coli 143 E.coli 164 Produce shigella like toxin Dysentery like diarrhea Sereny test-virulence test |
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Virulence factors of e.coli
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Endotoxin
Common pili K1 antigen |
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Diffuse aggregative e.coli (daec)
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Elongation of microvilli
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Anaerogenic e.coli or inactive e.coli
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(-)gas production
(-)lactose NM |
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Anaerogenic e.coli or inactive e.coli is prvsly calles...
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Alkalescens dispar
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Verotoxin
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Hemorrgaic colitis and hemolytic-uremic syndrome
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SLT
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Shiga like toxin
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Infetious disease associated with e.coli
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Intestinal infections
UTI Bacteremia Neonatal meningitis |
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MUG TEST
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4-methylumbelliferyl-b-d glucuronide escherichia coli broth medium(FC-MUG)
-standard analysis of water |
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E coli produces enzyme called...
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beta-D-glucuronidase
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Isolated in blood, spinal fluid, wound infections, feces
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E. Hermanii
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Wound infection
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E.vulneris
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Bacterial substance that exerts its effect intestinal tract
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Enterotoxins
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Gunshot to abdomen, growth in KCN
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E.fergusonii
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Intestines of cockroach
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E.blattae
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E.adecarboxylata
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---
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Enteropathogenic e.coli (epec)
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E.coli 0111
E.coli 0114 Mucous with blood |
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Enteroinvasive e.coli
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E.coli 0124
E.coli 143 E.coli 164 Produce shigella like toxin Dysentery like diarrhea Sereny test-virulence test |
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Enterotoxigenic e.coli(etec)
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E.coli 06
E.coli 08 E.coli 025 Traveler's diarrhea, turista belly delhi, cholera like toxin, montezuma's revenge, WATERY DIARRHEA |
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Enterohemmorhagic e.coli(ehec) or VTEC vero toxi e.coli
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E.coli 0157:H7
Shiga-like toxin BLOODY diarrhea Undercooked infected hamburger Causes HEMOLYTIC UREMIC SYNDROME |
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Severe manifestation of EHEC
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Hemolytic uremic syndrome
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Ehec is differentiated by...
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SMAC
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Replace lactose macconkey agar
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Sorbitol
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Enteroaggregative e.coli(eaec)
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Enteradherent e.coli or agglutinating e.coli
Binding of pili |
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Klebsiella characteristics
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Lactose fermenters, encapsulated(thick layer of polyssacharide) NM, mucoid colonies
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Klebsiella characteristics
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Lactose fermenters, encapsulated(thick layer of polyssacharide) NM, mucoid colonies
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#2 cause of uti
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K.pneumoniae
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Klebsiella characteristics
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Lactose fermenters, encapsulated(thick layer of polyssacharide) NM, mucoid colonies
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#2 cause of uti
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K.pneumoniae
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Common name of k.pneumoniae
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Friedlander's baccilus
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Old name of k.pneumoniae
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Bacillus mucosus capsulatus
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Characterized by emission of bloody sputum
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K.pneumoniae
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Characterized by emission of bloody sputum
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K.pneumoniae
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Causative agent of communitu acquired pneumonia
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K.pneumoniae
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Characterized by emission of bloody sputum
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K.pneumoniae
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Causative agent of communitu acquired pneumonia
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K.pneumoniae
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Virulence factor of klebsiella
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Polysaccharide capsule; R plasmis
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Characterized by emission of bloody sputum
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K.pneumoniae
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Causative agent of communitu acquired pneumonia
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K.pneumoniae
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Virulence factor of klebsiella
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Polysaccharide capsule; R plasmis
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Most resistant antibiotic of klebsiella
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Ampicillin
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Other Klebsiella genus
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Klebsiella oxytoca-
Klebsiella ozonae Ozena- Klebsiella rhinoscleromatis Klebsiella planticola Klebsiella granulomatis |
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Enterobacter species
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Enterobacter aerogenes
Enterobacter cloacae Enterobacter agglomerans Enterobacter gergoviae Enterobacter sakazakii Enterobacter taylorae |
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Enterobac lysine are all (+) except
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E.cloacae
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Yellow colonies
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Enterobacter aerogenes
Enterobacter cloacae Enterobacter agglomerans |
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Yellow colonies that intensifies at 25deg, DNASE(+), sorbitol(-)
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Enterobacter sakazakii
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Cancerogenous
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Enterobacter taylorae
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Normal flora of GIT
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Enterobacter aerogenes
Enterobacter cloacae |
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Similar to k.pneumoniae, only indole(+)
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K.oxytoca
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Sinus inection
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K.ozonae
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Disease of nasal mucosa, inactive strain of k.pneumoniae
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Ozena
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Cause granulomatous(nose and nasopharynx)
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K.rhinoscleromatis
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Environmental org which causes UTI and wound infection
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K.planticola
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Safety pin appearance
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K.granulomatis
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Enterobacter is formerly
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aerobacter
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Enterobacter characteristics
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Resembles k.pneumonia
Highly motile Urease (-) exc E. gergoviae |
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Differentiating points between klebsiella pneumoniae and enterobacter
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SIM; m-enterobac nm-k.pneu
Ornithine decarboxylase; (+)entero (-)klebsiella |
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Hafnia characteristics
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NLF, motile, ONPG, KCN(+)
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Hafnia characteristics
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NLF, motile, ONPG, KCN(+)
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Hafnia old name
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Enterobacter alvei
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Hafnia on mac
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Colorless after 24 hrs or slightly pink in 24 to 48 hrs
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Hafnia on EMB
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Lavender/colorless
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Hafnia on XLD
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Red/yellow/colorless with or wo black centers
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Hafnia on HEA, SSA
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Colorless
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Commensal of human GIT
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Hafnia alvei
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Serratia gen charac
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Motile
NLF sucrose fermenters |
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Hafnia characteristics
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NLF, motile, ONPG, KCN(+)
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3 enzyme to distinguish serratia
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DNASE
LIPASE GELATINASE |
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Hafnia old name
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Enterobacter alvei
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Hafnia on mac
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Colorless after 24 hrs or slightly pink in 24 to 48 hrs
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Hafnia on EMB
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Lavender/colorless
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Hafnia on XLD
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Red/yellow/colorless with or wo black centers
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Hafnia on HEA, SSA
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Colorless
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Commensal of human GIT
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Hafnia alvei
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Serratia gen charac
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Motile
NLF sucrose fermenters |
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serratia produces this pigment that is red non water soluble
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PRODIGIOSIN
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Serratia appers _____ on MAC
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Clear colonies
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Hafnia characteristics
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NLF, motile, ONPG, KCN(+)
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3 enzyme to distinguish serratia
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DNASE
LIPASE GELATINASE |
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Serratia members
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Serratia marcescens
Serratia liquifaciens Serratia rubidaea Serratia oforifera |
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Hafnia old name
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Enterobacter alvei
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Hafnia on mac
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Colorless after 24 hrs or slightly pink in 24 to 48 hrs
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Hafnia on EMB
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Lavender/colorless
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Hafnia on XLD
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Red/yellow/colorless with or wo black centers
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Hafnia on HEA, SSA
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Colorless
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Commensal of human GIT
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Hafnia alvei
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Serratia gen charac
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Motile
NLF sucrose fermenters |
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serratia produces this pigment that is red non water soluble
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PRODIGIOSIN
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Serratia appers _____ on MAC
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Clear colonies
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Red pigment at room temp
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Serratia marcescens
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Multiply in liver and spleen
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Salmonella typhi
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Commona name of Salmonella
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Eberth's disease
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Pathogenicity of Salmonella
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Salmonellosis
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Incubation period of Salmonella
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1-4 weeks
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Salmonella Types of disease
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enteric fever
Septicemia Gastroenteritis |
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Same as typhoid fever... S.typhi and s.paratyphi
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Enteric fever
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Salmonella cholerasuis
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Septicemia
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Salmonella typhimurim and Salmonella enteritidis
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Gastroenteritis
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SEROGROUP A (salmonella paratyphi)
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salmonella paratyphi A
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SEROGROUP B (salmonella paratyphi)
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salmonella paratyphi B/ salmonella scottmulleri
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Non pathogenic Serratia
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Serratia liquifaciens
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SEROGROUP C (salmonella paratyphi)
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salmonella paratyphi C/ salmonella hirschfeldii
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SEROGROUP D (salmonella paratyphi)
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salmonella typhi
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Antigens produced by salmonella paratyphi mo
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O-SOMATIC
H-FLAGELLAR Vi-CAPSULAR,CARRIER |
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Mode of transmission of salmonella paratyphi
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Ingestion of mo in contaminated food
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Indication of salmonellosis
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Severe headache
Continuous fever Diarrhea |
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Enterocolitis/gastroenteritis
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Salmonella gallinarum
Salmonella enteritidis Salmonella derby |
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Bacteremia
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Salmonella cholerasuis
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Enteric fever/typhoid fever
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Salmonella typhi
(+) rose spot on skin,abdomen,chest Fecal oral routes |
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Paratyphoid fever
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S.paratyphi
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Difference bet salmonella and shigella
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Gjb
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Red pigment
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Serratia rubidaea
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Two leading cause of salmonellosis
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S. Typhimurium and s. Enteritidis
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Salmonella on EMB AND MAC
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Colorless colonies
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Salmonella on SSA
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Colorless with black center colonies
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Salmonella on XLD
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Pink to red colonies with black center; BSA - green w black center
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Salmonella on BGA
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Pink white opaque surrounded by BRILLIANT RED MEDIUM
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Salmonella on SELENITE f broth
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Enrichment med for salmonella
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Difference bet salmonella and shigella
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Shigella is transmitted by
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Flies
Fingers Food Feces Water HIGHLY COMMUNICABLE |
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Natural habitat of Shigella
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Intestinal tract of humans
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Virulence factor of Shigella
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Shiga toxin ---- o antigen
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Musty pungent odor and racid potato like odor
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Serratia odorifera
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Incubation period of Shigella
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1-7 days
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Non mannitol fermenters
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Shigella dysenteriae
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Shigella dysenteriae
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Dysentery bacillus or shiga's bacillus
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2 types of toxin of Shigella dysenteriae
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Exotoxin -- neurotoxin
Enterotoxin -- similar to cholera toxin |
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Mannitol fermenters (NLF)
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Shigella flexneri
Shigella boydii |
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Shigella flexnerii
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Strong's bacillus or Gay bowel syndrome
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Shigella boydii
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Boyd's bacillus or newcastle-manchester bacillus
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Mannitol fermenters (LLf)
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shigella sonnei
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Shigella sonnei
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Sonne duval bacillus or sonne's bacillus
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ewing's classification of shigella
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Heheb
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Harmful human pathogen that causes UTI, wound infection, pneumonia
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Serratia marcescens
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Shigella sp causes...
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Bacillary dysentery which causes...
Watery feces w mucus flex, blood streaks, and plenty of pus cells |
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Intestinal content of reptiles
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Arizona hinshawii
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Sodium malonate medium
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Green in color, (+) result -- bluecolor
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Biochemically similar to e.coli
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Edwardsiella
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ewing's classification of shigella
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Found in aquatic animals reptiles frogs turtles and cause gastroenteritis and wound infections
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edwardsiella tarda
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Morphologically similar to e.coli and biochemically resembling salmonella
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Citrobacter
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Causes UTI and sepsis
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Citrobacter
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Citrobacter H2s (+)
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Citrobacter freundii
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Citrobacter H2s (-)
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Citrobacter Koseri
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Salmonella gen characteristics
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Pathogenic
Produce h2s |
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Accdng to kauffman antigenic scheme there are ___ species of Salmonella
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2000 sp of Salmonella
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Ewing proposes 3 species of Salmonella
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Salmonella typhosa/typhi
Salmonella cholerasuis Salmonella enteritidis |
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Major reservoir of Salmonella
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CHICKEN
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Checks disinfectant
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Salmonella serotype cholerasuis
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