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59 Cards in this Set
- Front
- Back
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Entero-
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dealing with intesting
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all have in common
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g-b
glucose fermenters oxidase - nitrite reducers not all lactose fermenters BA- gray mucoid fairly large and may be hemolytic |
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Lactose fermenters (LF)
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E. coli (green sheen on EMB, pink fried egg on MAC)
klebsiella (stringy, non motile) enterobacter arizona citrobacter yersinia (hold mac @ 25') |
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Non-lactose fermenters (NLF)
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salmonella
shigella edwardsiella serratia (red pigment 25") proteus (swarming -very motile) Providencia Morganella |
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Vibrio special media
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TCBS
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differentiate between Proteus & Providencia
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Urea
proteus- urea + providencia- urea - |
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3 urea +
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klebsiella
morganella proteus |
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3- NON motile
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yersinia
shigella klebsiella |
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3-PAD deaminase +
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proteus
providencia morganella R/A (red/yellow) |
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Ecoli-(LF)
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test: TSI= a/a + gas (Yellow/yellow)
LIA= k/k (purple/ purple) SIM= - + + IMViC= + + - - |
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serological
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o= somatic, cell wall, heat stable
k= envelop, capsule, heat liable h-flagella- motile bacteria |
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ETEC
EIEC EPEC EHEC |
montezuma's revenge
shigella like infections diarrhea in newborns o157h7 |
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ecoli disease
EHEC disease |
UTI= 90% non hospital, appendicitis, peritonitis, gallbladder infection, pneumonia, endocarditis, meningitis, wound infection, septicemia, cystitis
(HUS) caused by EHEC |
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Shigella (NLF) test
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TSI= k/a (red/ yellow)
LIA=k/a (purple/yellow) SIM= - - - Urea= - PA= - |
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shigella disease
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bacillary dysentary, fluid and electrolyte imbalance
NOTES: non manitol fermenters= s. dysenteiae mannitol fermenters= s. flexneri, s.boydii, s.sonnei (ONPG +) |
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Edwardsiella- NLF
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tsi= k/a +gas +h2s (red/black)
lia= k/k (purple/purple) SIM= + + + species: e. tarda |
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Salmonella-NLF (NON MOTILE) form endotoxins
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tsi= k/a =gas =h2s (red/black)
lia= k/k =h2s sim= + - + indole= - citrate= - urea= - ONPG= - |
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salmonella disease
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food poison- vomiting & diarrhea (s.enteritidis, s.typhi)
typhy fever= typhoid & paptyphid fever initial stage- septicemia blood culture- specimen of choice (1st 10 days) causitive agent febrile agglutination -used to ID antibodies in serum |
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S. typhi
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no gas, goatee h2s
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Enterobacter (LF)
Test |
tsi= a/a +gas (yellow/yellow)
Cit= + Sim= --+ urea= - Pa= - Lysine= +/- odc= + |
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enterobacter disease
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UTI, speticemia, opportunistic
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enterobacter species
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e. cloacae
e. aerogenes e. agglomerans |
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Serratia (NLF)
test Chromogenis- red pigment at 25'C |
tsi= k/a (red/ yellow)
Cit= + sim= - - + urea= - lia= k/k (purple/purple) |
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serratia disease
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UTI, URT
septicemia: burn and chemo patients diabetics with catheters and inhalation therapy VERY RESISTANT to antibiotics |
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Proteus (NLF)
test SWARMING (very motile) |
tsi= k/a +gas + h2s
lia= red/a +h2s (red/black) sim= + + +, (+ - +) urea= + Pa= + |
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proteus disease
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UTI, peritonitis (in ruptured appendix)
ear & eye infections, smells bad |
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differentiate between p. mirabilis and p. vulgaris
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Indole
p.mirabilis = - p.vulgaris = + |
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arizona (LF) slowly
test |
tsi= k/a +gas +h2s (red/black)
lia= k/k +h2s (purple/black) ONPG= + *DOES NOT INVADE BLOOD STREAM species a. hinshawii |
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Citrobacter (LF) slowly
test |
Tsi= k/a +gas some h2s (red/ black) when h2s is present
ONPG= + disease: UTI, opportunistic |
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klebsiella (LF) NON MOTILE mucoid (stringy)
test |
tsi= a/a +gas (yellow/yellow)
lia= k/k (purple/ purple) decarboxylate + sim= - - - urea= + pa= - IMViC= - - + + odc= - |
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Klebsiella pneumo
test disease |
Friedlanders Bacillus
IMViC= - - + + pneumonia , severe UTI |
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klebsiella oxytoca
test disease |
IMViC= + - + +
noscomial UTI |
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differentiate between K. pneumo & k. oxytoca
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indole
k.pneumo= - k. oxytoca = + |
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Providencia (NLF)
test species: p. alcalifaciens, p.stuartii |
tsi= k/a (red/yellow)
lia= R/a (red/yellow) DEAMINASE + cit= + Urea= - sim+ - + + |
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differentiate between Providencia and Proteus
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Urea
proteus= + providencia = - |
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Morganella (NLF)
test |
tsi= k/a (red/yellow)
lia= r/a (red/yellow) DEAMINASE + cit= - sim= - + + urea= + |
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Yersinia (LF)
requires enriched media CIN agar 3-types |
y.pestis
y. enterolytica y. pseudotuberculosis |
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y. pestis
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non-motile, causes plague
vector- rat flea/ dead carcass disease: bubonic plague (swollen lymph nodes & fever), sepsis, pneumonia follow plague pneumonia_ black death, untreated 50-60 % death VERY INFECTIOUS |
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y. enterolytica
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bipolar staining, implicated in enlarged lymph nodes
disease= gastroenteritis, abscesses, fatal septicemia, may mimic appendicitis HOLD mac plate xtra 24hours @ RT (tiny peach colonies) |
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y. pseudotuberculosis
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gram - tiny pleomorphic rods,
affects lymph nodes, fatal septicemia, mimics appendicitis |
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Campylobacter
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most common etiologic agent in US
curved rods (seagull wings) microaerophilic & Capnophilic= ^ co2 (10%), o2 (5%) GI tract- poultry, dogs, cats, sheep, cattle special media= campy media (BA with antibiotics) 42'C (24hrs- 48 hrs, gray pinkish- yellowish gray slightly mucoid, may have tailing effect |
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campylobacter
transmitted and disease campylobacter species |
transmitted by contaminated food
disease: gastroeneritis, meningitis, endocarditis, septic arthritis c. jejuni: common in gastroenteritis |
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campylobacter test
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oxidase +
catalase + motility + (darting motion on wet prep) |
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Helicobacter
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used to be campylobacter
g- curved rods primary habitat: human gastric mucosa STRONGLY= urease + |
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Species of helicobacter
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h. pylori; gastric and peptic ulcers and may be associated with gastric cancer.
stomache biopsy, blood and breath tested for urease causative agent most individuals tolerate organisms for decades without symptoms. |
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Vibrio- halophilic (salt loving), fermentative
special media 3 species |
Oxidase +
TCBS media v. cholera v. paphemolyticus v. vulnificus |
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Vibrio cholera
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can grow w/o NaCL
yellow colony on TCBS Disease: asiatic choler, epidemic cholera can cause acute diarrhea (rice water stools) death due to severe dehydration |
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Vibrio paphemolyticus
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requires NaCL, lysine decarboylase +
green colony on TCBS disease: gastroenteritis & soft tissue infections leading cause of GI illness in Japan (uncooked seafood) |
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Vibrio vulnificus
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requires NaCL, lysine decarboylase + ONPG +
most colonies green on TCBS disease: primary septicemia and soft tissue infections *seen in marine environments, and liver conditions |
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Methyl Red
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acid end products
mixed acid pathways |
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VP (voges proskauer)
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neutral end products
butylene glycol pathway |
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Citrate
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determines the ability of an organism to utilize sodium citrate as its only carbon source.
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tet used to differentiate Salmonella & Proteus
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LIA & PAD
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What carbohydrate does the test MR & VP utilize in different pathways
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Glucose
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Enterobacter cloacae
test |
IMViC= - - + +
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Yersinia enterocolitica grows in cold and is motile when
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25'C it is motile and @ 35'C it is nonmotile
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E. Coli o157 h7 is unable to ferment lactose
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false it is unable to ferment Sorbital
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Campylobacter jejuni
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microaerphilic
seagull wings and darting motility @ 42'C |
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E. Coli O157 H7 produce what
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verotoxin and shiga toxin
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