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11 Cards in this Set
- Front
- Back
- 3rd side (hint)
Bacillus cereus
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BCA
CIT: coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole LIA LIA control MAC MEA MSA MOT NA O/F test ONPG staphytect MR VP TBA TSIA urea GPPW XLD |
Gram positive
Rod |
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Enterobacter aerogenes
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BCA
BP catalase CIT: blue medium. positive. can metabolise citrate as sole carbon source. produces the citritase enzyme which metabolises citrate to oxaloacetate and acetate. coagulase DNA EYA glucose fermentation Haemolysis HBA indole: transparent. negative. cannot metabolise tryptophan to indole. does not produce the tryptophanase enzyme. LIA LIA control MAC: red colonies. positive. bile tolerant and lactose fermenting. production of both beta galactasidase and permease enzymes. MSA MOT: spread of growth in semi solid medium from point of innoculation. motile. flagella. NA O/F test ONPG: bright yellow medium. positive. lactose fermenting by production of the beta galactasidase enzyme. staphytect MR: yellow medium. negative. no acidic products following glucose metabolism. VP: red medium. positive. neutral products following glucose metabolism. TSIA urea: pale yellow medium. negative. does not produce urease enzyme. cannot metabolise urea to ammonia. GPPW |
Gram negative
Rod |
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Escherichia coli
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BCA
CIT: green medium. negative. cannot metabolise citrate as sole carbon source. does not produce citritase enzyme. coagulase DNA EYA glucose fermentation Haemolysis HBA indole: red surface layer. positive. produces the tryptophanase enzyme which metabolises tryptophan to indole. LIA LIA control MAC: red colonies. positive. bile tolerant and lactose fermenting. production of both beta galactasidase and permease enzymes. MSA MOT: spread of growth in semi solid medium from point of innoculation. motile. flagella. NA O/F test ONPG: bright yellow medium. positive. lactose fermenting by production of the beta galactasidase enzyme. MR: red medium. positive. acidic products following glucose metabolism. VP: yellow medium. negative. no neutral products following glucose metabolism. TSIA urea: pale yellow medium. negative. does not produce urease enzyme. cannot metabolise urea to ammonia. GPPW |
Gram negative
Rod |
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Klebsiella pneumoniae
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BCA
BP catalase CIT: blue medium. positive. can metabolise citrate as sole carbon source. produces the citritase enzyme which metabolises citrate to oxaloacetate and acetate. coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole: transparent. negative. cannot metabolise tryptophan to indole. does not produce the tryptophanase enzyme. LIA LIA control MAC: red colonies. positive. bile tolerant and lactose fermenting. production of both beta galactasidase and permease enzymes. MEA MSA MOT: no spread of growth from point of innoculation. non motile. NA O/F test ONPG: bright yellow medium. positive. lactose fermenting by production of the beta galactasidase enzyme. staphytect MR: red medium. positive. acidic products following glucose metabolism. VP: yellow medium. negative. no neutral products following glucose metabolism. TBA TSIA urea: pink medium. positive. produces the urease enzyme which metabolizes urea to ammonia. GPPW XLD |
Gram negative
Rod |
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Proteus mirabilis
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BCA
BP catalase CIT: blue medium. positive. can metabolise citrate as sole carbon source. produces the citritase enzyme which metabolises citrate to oxaloacetate and acetate. coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole: transparent. negative. cannot metabolise tryptophan to indole. does not produce the tryptophanase enzyme. LIA LIA control MAC: brown colonies. negative. bile tolerant and non lactose fermenting. does not produce both the beta galactasidase and permease enzymes. MEA MSA MOT: spread of growth in semi solid medium from point of innoculation. motile. flagella. NA O/F test ONPG: pale yellow medium. negative. non lactose fermenting. does not produce the beta galactasidase enzyme. staphytect MR: red medium. positive. acidic products following glucose metabolism. VP: yellow medium. negative. no neutral products following glucose metabolism. TBA TSIA urea: pink medium. positive. produces the urease enzyme which metabolizes urea to ammonia. GPPW XLD |
Gram negative
Rod |
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Pseudomonas sp.
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BCA
BP catalase CIT coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole LIA LIA control MAC MEA MSA MOT NA O/F test ONPG staphytect MR VP TBA TSIA urea GPPW XLD |
Gram negative
Rod |
|
Salmonella typhimurium
|
BCA
BP catalase CIT: blue medium. positive. can metabolise citrate as sole carbon source. produces the citritase enzyme which metabolises citrate to oxaloacetate and acetate. coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole: transparent. negative. cannot metabolise tryptophan to indole. does not produce the tryptophanase enzyme. LIA LIA control MAC: brown colonies. negative. bile tolerant and non lactose fermenting. does not produce both the beta galactasidase and permease enzymes. MEA MSA MOT: no spread of growth from point of innoculation. non motile. NA O/F test ONPG: pale yellow medium. negative. non lactose fermenting. does not produce the beta galactasidase enzyme. staphytect MR: red medium. positive. acidic products following glucose metabolism. VP: yellow medium. negative. no neutral products following glucose metabolism. TBA TSIA urea: pale yellow medium. negative. does not produce urease enzyme. cannot metabolise urea to ammonia. GPPW XLD |
Gram negative
Rod |
|
Shigella dysenteriae
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BCA
BP catalase CIT: green medium. negative. cannot metabolise citrate as sole carbon source. does not produce citritase enzyme. coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole: transparent. negative. cannot metabolise tryptophan to indole. does not produce the tryptophanase enzyme. LIA LIA control MAC: brown colonies. negative. bile tolerant and non lactose fermenting. does not produce both the beta galactasidase and permease enzymes. MEA MSA MOT: no spread of growth from point of innoculation. non motile. NA O/F test ONPG: bright yellow medium. positive. lactose fermenting by production of the beta galactasidase enzyme. staphytect MR: red medium. positive. acidic products following glucose metabolism. VP: yellow medium. negative. no neutral products following glucose metabolism. TBA TSIA urea: pale yellow medium. negative. does not produce urease enzyme. cannot metabolise urea to ammonia. GPPW XLD |
Gram negative
Rod |
|
Staphylococcus aureus
|
BCA
BP catalase CIT coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole LIA LIA control MAC MEA MSA MOT NA O/F test ONPG staphytect MR VP TBA TSIA urea GPPW XLD |
Gram positive
Cocci Grape clusters |
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Vibrio sp.
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BCA
BP catalase CIT coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole LIA LIA control MAC MEA MSA MOT NA O/F test ONPG staphytect MR VP TBA TSIA urea GPPW XLD |
Gram negative
Curved rod |
|
BCA
BP catalase CIT coagulase CTA DNA ELEK EYA glucose fermentation Haemolysis HBA indole LIA LIA control MAC MEA MSA MOT NA O/F test ONPG staphytect MR VP TBA TSIA urea GPPW XLD |
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