Endothelial Cells And Platelets

Front 1

Endothelial products

Back 1

1.) collagen
2.) endothelin-1
3.) Tissue Thromboplastin

Front 2

Collagen

Back 2

plts. intrinsic, and extrinsic pathways

Front 3

endothelin-1

Back 3

vasoconstriction

Front 4

tissue thromboplastin

Back 4

tissue phospholipoprotein, activates F 7 (extrinsic), capable of converting prothrombin to thrombin

Front 5

Prothrombotic

Back 5

1.) Plt.-activating factor
2.) Tissue factor
3.) von Willebrand factor
4.) plasminogen activator
5.) Factor V

Front 6

Plt activating factor

Back 6

in endothelium

Front 7

tissue factor

Back 7

extrinsic pathway

Front 8

vWf

Back 8

coagulation factor produced in endothelial cells

Front 9

plasminogen activator

Back 9

clot removal, stops and break clots

Front 10

factor V

Back 10

coagulation factor produced in endothelial cells, also in
megakaryocytes

Front 11

Antithrombotic

Back 11

1.) Prostacyclin
2.) Thrombomodulin
3.) Tissue plasminogen activator (tPA)
4.) Urokinase
5.) Heparin-like molecules

Front 12

prostacyclin

Back 12

prevents plt. adhesion and aggregation

Front 13

thrombomodulin

Back 13

starts sequence, binds protein C, S and thrombin

Front 14

tissue plasminogen activator (tPA)

Back 14

fibrinolysis

Front 15

Urokinase

Back 15

fibrinolysis

Front 16

Disrupted Endothelium

Back 16

- activates factor 12 (intrinsic)
- exposes collagen
- vasoconstriction by endothelin-1 and angiotensin 2
- fibrinolysis starts at the same time as coagulation to balance

Front 17

Glycocalyx

Back 17

– surface coat, unique coating, protein coat mediates adhesion and allows adherence of coagulation proteins

Front 18

Membrane

Back 18

- 2 proteins exposed for adhesion and aggregation
* glycoprotein Ib
* glycoprotein IIb/IIIa
- open canalicular system

Front 19

glycoprotein Ib

Back 19

binds vWF

Front 20

glycoprotein IIb/IIIa

Back 20

binds fibrinogen

Front 21

Open canalicular system

Back 21

canals run through plt, delivers contents to
surface

Front 22

Granules

Back 22

- Alpha granules
- Dense granules
- Lysosomes
- Factor 5, plt factor 3, and glycogen

Front 23

Alpha granules

Back 23

1.) plt factor 4
2.) plt growth factor
3.) beta thromboglobulin
4.) vWF
5.) thrombospondin

Front 24

Dense granules

Back 24

1.) ADP and ATP
2.) calcium
3.) serotonin

Front 25

lysosomes

Back 25

hydrolase enzymes

Front 26

Adhesion

Back 26

- plts adhere to collagen mediated by vWF
1.) filament contract, turns disk to sphere, pseudopods form
2.) contraction and release epinephrine and serotonin promotes vasoconstriction
- alpha granules go out and in
- release – dense ADP/ATP accelerates plt aggregation, irreversible, adhesion is reversible
3.) glycoproteins IIb/IIIa and Ib appears on the membrane, binds fibrinogen and vWF, increases adhesiveness and helps initiate aggregation

Front 27

Bernard-Soulier Syndrome

Back 27

- hereditary
- cell membrane disorder
- glycoprotein Ib is absent, Ib is the primary receptor for vWF
- have difficulty ADHERING, vWF is necessary for adhesion to subendothelial tissue
- aggregation studies normal except for ristocetin

Front 28

Glanzmann’s thrombasthenia

Back 28

- cell membrane disorder
- lacking IIb/IIIa
- decreased fibrinogen binding
- AGGREAGATION affected, aggregation studies abnormal

Front 29

Aggregation

Back 29

requires the release reaction to happen first

Front 30

Which of the following factors is found in the endothelial cells?
A.) Factor V
B.) Factor XII
C.) Factor I
D.) Factor XIII

Back 30

A.) Factor V

Front 31

What helps prevent platelets from adhering to normal endothelial cells?
A.) collagen
B.) glycoprotein Ib
C.) glycoprotein IIb/IIIa
D.) vWF
E.) prostacyclin

Back 31

E.) prostacyclin

Front 32

Plt factor 4, beta thromboglobulin, thrombospondin, and plt growth factor are all contained in the:
A.) alpha granules
B.) dense granules
C.) lysosomes
D.) cytoplasm

Back 32

A.) alpha granules

Front 33

In Bernard Soulier Syndrome, this glycoprotein is decreased or absent:

Back 33

Ib

Front 34

The primary receptor for fibrin/fibrinogen

Back 34

IIb/IIIa

Front 35

The primary platelet receptor responsible for platelet adhesion

Back 35

Ib

Front 36

The cellular ultrastructural component(s) unique to platelets is/are:
A.) cytoplasmic membrane
B.) glycocalyx
C.) mitochondria
D.) lysossomes

Back 36

B.) glycocalyx

Front 37

Platelet aggregation studies can help identify vW disease using which of the following reagents?
A.) ADP
B.) Epinephrine
C.) Collagen
D.) Ristocetin

Back 37

D.) ristocetin

Front 38

Aspirin blocks the cyclooxygenase pathway by:
A.) attaching an -COOH to thrombomodulin
B.) by deacytlyating antinomysin
C.) by acetlyating prostaglandin synthetase
D.) blocking the synthesis of kallekrein from prekallekrein

Back 38

C.) by acetlyating prostaglandin synthetase

Front 39

COX-2 inhibitors may put users at risk of a cardiac event because:
A.) it inhibits production of prostacyclin
B.) it inhibits the production of thromboxane a2
C.) it does NOT inhibit the inflammatory response
D.) it protects the stomach lining

Back 39

A.) it inhibits production of prostacyclin

Front 40

Glycoprotein Ib in the platelet membrane primarily binds to:
A.) fibrinogen
B.) plt factor 3
C.) beta thromboglobulin
D.) vWf

Back 40

D.) vWf

Front 41

Prostaglandin forms what?

Back 41

thromboxane A2 and prostacyclin

Front 42

Thromboxane A2

Back 42

required for platelet aggregation

Front 43

Prostacyclin

Back 43

inhibits platelet aggregation and adhesion

Front 44

COX-1

Back 44

protects the stomach lining and is responsible for the production of thromboxane A2

Front 45

COX-2

Back 45

- produces prostacyclin
- triggers the pain and inflammatory response
- COX-2 inhibitor contributes to pain relief and reduction of inflammation, also decrease in prostacyclin