Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
30 Cards in this Set
- Front
- Back
|
structure => inheritance
|
nucleotide bases -> information
base complementarity -> replication |
|
|
Griffith
|
R -> S => xfm
|
|
|
Avery et al.
|
DNase -> -S
|
|
|
Hershey-Chase
|
phage 32P DNA -> cell
35S protein -> phage ghost |
|
|
nucleotide
|
phosphate + deoxyribose + base
|
|
|
purine
|
AG
|
|
|
pyrimidine
|
TC
|
|
|
Chargaff's rules
|
T + C = A + G
T = A C = G A + T ~= G + C |
|
|
Franklin
|
X-ray diffraction:
DNA long, skinny, parallel length-wise |
|
|
backbone
|
-- 5' - phosphodiester - 3' --
antiparallel |
|
|
pairing
|
1' - base---base - 1'
|
|
|
stacking
|
plate -> stability
|
|
|
grooves
|
major (most ptn assoc)
minor |
|
|
replication models
|
* semiconservative - P-D P-D
conservative - P-P D-D dispersive - PD-PD PD-PD |
|
|
Meselson-Stahl
|
15N P + 14N medium -> 14/15 D + 14/14 D'
CsCl gradient -> banding |
|
|
replication fork
|
Cairns: cold DNA + hot cell -> seam
|
|
|
DNA polymerase
|
base--3' O <- 5' P--complement
|
|
|
pol III
|
catalyzes synthesis at fork
|
|
|
replication strands
|
--- 3'---5' leading <-
--- 5'---3' lagging -> |
|
|
Okazaki fragment
|
short stretches at lagging strand
|
|
|
lagging strand synthesis
|
primase -> 5'--3' RNA primer
pol III 3'---> primer---> pol III ---------> ---> DNA ligase --------> |
|
|
pol exonuclease activity
|
proofreading
|
|
|
replisome
|
pol III holoenzyme + accessory ptns
|
|
|
pol III holoenzyme
|
leading + lagging catalytic cores
|
|
|
beta clamp
|
pol III distributive -> processing
|
|
|
helicase
|
base--/ /--base
|
|
|
SSB proteins
|
prevent helix reformation
|
|
|
topoisomerase
|
cut, rejoin to relax supercoils
|
|
|
DnaA
|
initiate replication:
recognize oriC bind to DnaA box melt ATs to unwind recruit replisome |
|
|
helicase
|
bind and slide 5' -> 3' to unzip helix
|
