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36 Cards in this Set
- Front
- Back
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What are the 2 sugars involved in the Nucleotide?
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1) Ribose 2) Deoxyribose |
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What are the bases involved in the Nucleotide?
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1) Purine (AG) 2) Pyramidine (CUT) |
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The base of the nucleotide is connected to _______ carbon via _______ bond.
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2) Glycosidic |
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What are the 3 phosphates involved in the Nucleotide?
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1) Alpha 2) Beta 3) Gamma |
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What is an example of a cancer predisposition disorder?
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Bloom Syndrome |
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What is an example a RecQ DNA Helicase Mutation?
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Bloom Syndrome |
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The __________ is defective in Bloom's syndrome and is thought to be a part of the complex that unwinds DNA as two replication forks meet.
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DNA Helicase |
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The DNA Polymerase EPSILON and DELTA have ________ activity.
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Exonuclease |
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DNA polymerase can recognize the incorporation of __________ due to lack of _________.
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1) Wrong Bases 2) Watson Crick Base Pairing |
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DNA Polymerase REMOVES _____________.
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Wrong Base |
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Nucleosome = ________+________
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Nucleosome has ______ Histone proteins (_____X each typical histone proteins).
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2) 2 |
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_______bp DNA wrapped _________ around the 8 histone proteins. |
2) Twice |
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Nucleosomes are separated by _______bp known as ________. |
2) Linker DNA |
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What does NAP stand for?
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Nucleosome Assembly Protein |
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The Nucleosome Assembly is broke down into 3 facts including: 1) Histones made by _________. 2) Histones are _________. 3) _______ facilitates nucleosome assembly. |
2) Acetylated 3) NAP-1 |
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The acetylation of histones allows the histone to bind ________ to enter _______.
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2) Nucleus |
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The acetylation does NOT impact ______/______ with DNA.
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Gene Expression/Interaction |
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Telomerase = ____________
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Ribonucleopeptide |
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Ribonucleopeptide = ________ + ________
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Polypeptide + RNA template |
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Telomerase recognizes _____' _____ REPEATS.
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3' Terminal |
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Telomerase extends 3' end using _______.
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RNA template |
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Telomerase adds _________.
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Deoxynucleotides |
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What inhibits cyclin D1--CDK4 complex that leads to negative transcription? |
P16 |
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Which complex goes from: Kinase --> phosphorylate Rb No P16 --> active --> RB-Pi --> E2F Active --> positive transcription |
Cyclin D1-CDK4 Complex
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During the Cyclin D1-CDK4 Complex, Kinase --> ________ |
Kinase --> Phosphorylate Rb |
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During the Cyclin D1-CDK4 Complex, No P16 --> ________ --> ________ --> _________ |
No P16 --> active --> Rb-Pi --> E2F
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During the Cyclin D1-CDK4 Complex, Active --> ________ |
Active --> + Transcription
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Transactivator + transcription of proteins needed for S-phase |
E2F |
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HYPO-phosphorylated --> inhibit E2F --> - transcription HYPER-phosphorylated --> unbind E2F --> + transcription |
Rb |
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HYPO-phosphorylated --> _______ --> _______. HYPER-phosphorylated --> _______ --> _______. |
HYPO-phosphorylated --> inhibit E2F --> - transcription HYPER-phosphorylated --> unbind E2F --> + transcription |
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A CDK inhibitor. |
p16 |
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A G1-CDK |
Cyclin D1-CDK4 Complex |
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An inhibitor of a gene regulatory protein. |
Rb |
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A gene regulatory protein. |
E2F |
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What are the steps of PCR? |
1) Isolate DNA 2) Heat Denature (separate) dsDNA 3) Cool --> primers anneal 4) DNA Synthesis 5) Repeat |
