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11 Cards in this Set
- Front
- Back
Replication
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Process by which one helix of DNA gives rise to two identical helices.
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Semi-conservative; Example
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Each new helix is composed of one parent strand and one daughter strand; DNA
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Transcription
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First step in transforming DNA into a protein.
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RNA contains
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Adenine, guanine, cytosine, and uracil.
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What are the three types of RNA? What are their functions?
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1.) Messenger RNA- Blueprint for constructing protein
2.) Transfer RNA- carries amino acids around in the cytoplasm 3.) Ribosomal RNA- forms ribosomes where protein synthesis occurs |
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Translation:
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Process by which the message that contains mRNA is translated into strand of amino acid.
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Techniques that involve analysis of DNA
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1) Restriction Enzymes
2) Agarose gel |
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Restriction Enzymes; Example
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Cut DNA into small pieces, other RE's cut even larger pieces; Example: Eco Ri: cuts DNA into 750,000 fragments
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Restriction Fragment Length Polymorphisms
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Pattern of number and fragment size.
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What is the process of Restriction Length Polymorphisms?
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First, they are visualized through agarose gel electrophoresis, DNA is cut into small pieces and is placed in agarose gel and then subjected to electrical current. The negatively charged DNA move towards positive electrode.
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Polymerase Chain Reaction
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Small amounts of DNA that can be amplified for analysis.
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