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5 Cards in this Set
- Front
- Back
- 3rd side (hint)
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OXIDASE TEST |
Adding OXIDASE REAGENT |
Pseudomonads or Enteric |
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Lactose Fermentation PR Lactose Broth
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Normal Flora or Possible Pathogen |
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Lactose Fermentation MAC (MacConkey's Agar) |
Growth ➡ bile salt/dye tolerant No Growth ➡ bile salt/dye intolerant
Pink Growth ➡ Lactose Fermenter Colorless Growth ➡ Lactose NonFermenter
(Lactose Fermenter = Gram+) |
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Sulfide & Indole SIM agar deeps |
Black ➡ produces H2S gas Yellow ➡ H2S negative Yellow w/Red top ➡ Indole pos. Black or plain Yellow ➡ Indole neg. |
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Acid end vs. Non-Acid end MR-VP broth |
⤵Methyl Red (MR) Test: Stayed Yellow = Non-acid (negative) Turned Red = Acid (positive)
⤵ Voges-Proskauer (VP) Test: Turned Red = positive (Acetoin>Butanediol) Stayed Yellow = negative
(If it is positive in one test it should be negative in the other test, and vice versa) |
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