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14 Cards in this Set
- Front
- Back
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What do you need to grow/ID a virus?
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grow in a cell culture
detect specific proteins specific genome sequences serology good patient history |
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Best time to collect a viral sample is:
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early in acute phase when virus is being shed
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Viral Storage/Transport
1. VTM 2. store sample on _____; but do not _____ |
1. protein, pH buffer, Antimicrobials
2. Ice; freeze |
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Cytology - characteristic changes in cell morphology:
1. Syncytia 2. inclusion bodies 3. Owl eye 4. Negri bodies Good for initial diagnosis, but not conclusive |
1. multinucleated giant cells
2. histological changes in cells 3. basophilic nuclear inc body - CMV 4. in cytoplasm; processes of neurons |
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Diploid cell line
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culture of single cell type that can be passed a large but finite number of times
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Tumor Cell Lines/Immortalized
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can be passed continuously w/o senescing
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Plaques
heterologous interference |
visible colonies of lysed cells
one virus prevents replication of another known virus |
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Hemagglutination Inhibition
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ID of a virus by blocking/inhibiting agglutination w/specific antibodies against virus
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Immunofluorescence
Direct Indirect |
cells are detected by antibody interactions
mono-specific Ab's conjugated to fluorescent molecule bind to viral protein mono Ab's bound to cells with viral surface antigens |
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Enzyme Immunoassay
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enzyme-conjugated Ab is used; substrate is converted to chromogen that marks infected cells
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ELISA
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antigen is on bead; virus-specific Ab's are captured; quantitative is sample is serially diluted and compared against known concentrations
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Radioimmunoassay
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use radiolabeled antibody/antigen
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Latex Agglutination
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latex beads used to detect Ag/Ab in patient's sample
beads form clumps in +rxn |
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Shell Vial
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sample is centrifuged onto cells grown on glass cover-slip
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