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9 Cards in this Set
- Front
- Back
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Kind of questions that would ask you to test the gene expression at DNA level? |
Is there a chromosomal level change? Is my gene of interest present? Is there a sequence level change? Is my model close enough related? If our gene have been knocked out or mutated or if our transgene have been integrated into the genome? |
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Techniques used to test gene expression at DNA level |
Karyotype ( chromosome level) Southern hybridisation DNA sequencing PCR |
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Kind of questions that would lead you for RNA level of testing |
The expression of gene of interest at a particular time?
Are there differences in transcript level of my gene between these two tissues or at these two times? |
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Techniques for RNA testing? |
Northern blot RT-PCR Microarray Cluster and heat map analysis RNA in situ hybridisation |
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Techniques for RNA testing? |
Northern blot RT-PCR Microarray Cluster and heat map analysis RNA in situ hybridisation |
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Kind of questions that can ask you to test protein level? |
Is there protein being produced by my gene of interest? - when and where is it produced?
Where in the cell is the protein localised? |
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Techniques for RNA testing? |
Northern blot RT-PCR Microarray Cluster and heat map analysis RNA in situ hybridisation |
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Kind of questions that can ask you to test protein level? |
Is there protein being produced by my gene of interest? - when and where is it produced?
Where in the cell is the protein localised? |
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Tests for testing at protein level? |
Western blot Immunohistochemistry Immunofluoresence Confocal images |
