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50 Cards in this Set

  • Front
  • Back
what edition? listeria
at first volume 2, then volume 3 firmicutes
What other genera?
brochothrix, erysiplothix and Carynanon
Carynanon
not clinically important, multicellular - forms trichomes
Domain, phylum, etc. listeria
bacteria, firmicutes, bacilli, bacillales, Listeriaceae
Listeria contains what 2 genera?
listeria and Brochothrix
How many species of listeria?
6 species
L. ivanovii
extremely rare human pathogen. Ruminent animals
L. seeligeri
rare human pathogen
L. innocua
non-pathogenic
L. monocytogenes has ____ serotypes based on what?
13 serotypes based on somatic (O) and flagellar (H) anitgens
Serotype __ cause the majority of L. monocytogenes infections
4b
What are the 3 lineages based on?
three virulence genes
Tell about the three lineages
Lineage 1 - most clinical strains. Lineage 2 - some clinical strains. Lineage 3 - mostly animal pathogens
Gram ?, morphology, spores?, capsules?, Acid fast?
Gram +, coccoballiary rods, non-encapsulated, non- acid fast
Optimum growth, motile, areobic?, catalase, oxidase
optimum temp 30-37C, Motile at 10-25, Facultative anaerobes, Catalase +, Oxidase -
Listeria and food
tolerate high salt, low pH, multiply at fridge temp, biolfilms on food-processing
Three lifestyles of L. monocytogenes
i - intracellular. ii extracular, iii biofilm. change from ii to i by PrfA
Lifecycle of L. monocytogenes
1. adhesion to phagosome 2. escape phagosome 3. intracellular multiplication 4. actin-induced motility 5. neighboring cells repeat
Listeria live in host cells so its...
sheltered from the immune system
Virulence factors of L. monocytogenes
LIL_PA. Listeriolysin O, Listeria Pathogencity Island-1 (LIPI-1), Internalins A and B, Phospholipase C, Actin-Polymerizing protein A
Listerosis illness
epidemic or sporadically. Hight hospitalization rate of food illnesses, high mortality rate (20-30%)
Listeria grow well on
blood and chocolate agar and Fraser broth
Specimen growth of listeria
1 specimen :9 broth. incubate at 4C for days to weeks. Subculture frequently until recovery
Only 3 species are Beta hemolytic
L. monocytogenes. L. ivanovii, L. seeligeri (non-pathogenic)
Primers in Listeria
hly, fla, ActA, InlA
what edition? listeria
at first volume 2, then volume 3 firmicutes
What other genera?
brochothrix, erysiplothix and Carynanon
Carynanon
not clinically important, multicellular - forms trichomes
Domain, phylum, etc. listeria
bacteria, firmicutes, bacilli, bacillales, Listeriaceae
Listeria contains what 2 genera?
listeria and Brochothrix
How many species of listeria?
6 species
L. ivanovii
extremely rare human pathogen. Ruminent animals
L. seeligeri
rare human pathogen
L. innocua
non-pathogenic
L. monocytogenes has ____ serotypes based on what?
13 serotypes based on somatic (O) and flagellar (H) anitgens
Serotype __ cause the majority of L. monocytogenes infections
4b
What are the 3 lineages based on?
three virulence genes
Tell about the three lineages
Lineage 1 - most clinical strains. Lineage 2 - some clinical strains. Lineage 3 - mostly animal pathogens
Gram ?, morphology, spores?, capsules?, Acid fast?
Gram +, coccoballiary rods, non-encapsulated, non- acid fast
Optimum growth, motile, areobic?, catalase, oxidase
optimum temp 30-37C, Motile at 10-25, Facultative anaerobes, Catalase +, Oxidase -
Listeria and food
tolerate high salt, low pH, multiply at fridge temp, biolfilms on food-processing
Three lifestyles of L. monocytogenes
i - intracellular. ii extracular, iii biofilm. change from ii to i by PrfA
Lifecycle of L. monocytogenes
1. adhesion to phagosome 2. escape phagosome 3. intracellular multiplication 4. actin-induced motility 5. neighboring cells repeat
Listeria live in host cells so its...
sheltered from the immune system
Virulence factors of L. monocytogenes
LIL_PA. Listeriolysin O, Listeria Pathogencity Island-1 (LIPI-1), Internalins A and B, Phospholipase C, Actin-Polymerizing protein A
Listerosis illness
epidemic or sporadically. Hight hospitalization rate of food illnesses, high mortality rate (20-30%)
Listeria grow well on
blood and chocolate agar and Fraser broth
Specimen growth of listeria
1 specimen :9 broth. incubate at 4C for days to weeks. Subculture frequently until recovery
Only 3 species are Beta hemolytic
L. monocytogenes. L. ivanovii, L. seeligeri (non-pathogenic)
Primers in Listeria
hly, fla, ActA, InlA