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50 Cards in this Set
- Front
- Back
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what edition? listeria
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at first volume 2, then volume 3 firmicutes
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What other genera?
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brochothrix, erysiplothix and Carynanon
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Carynanon
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not clinically important, multicellular - forms trichomes
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Domain, phylum, etc. listeria
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bacteria, firmicutes, bacilli, bacillales, Listeriaceae
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|
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Listeria contains what 2 genera?
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listeria and Brochothrix
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How many species of listeria?
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6 species
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L. ivanovii
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extremely rare human pathogen. Ruminent animals
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L. seeligeri
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rare human pathogen
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L. innocua
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non-pathogenic
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L. monocytogenes has ____ serotypes based on what?
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13 serotypes based on somatic (O) and flagellar (H) anitgens
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Serotype __ cause the majority of L. monocytogenes infections
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4b
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What are the 3 lineages based on?
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three virulence genes
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Tell about the three lineages
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Lineage 1 - most clinical strains. Lineage 2 - some clinical strains. Lineage 3 - mostly animal pathogens
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Gram ?, morphology, spores?, capsules?, Acid fast?
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Gram +, coccoballiary rods, non-encapsulated, non- acid fast
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Optimum growth, motile, areobic?, catalase, oxidase
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optimum temp 30-37C, Motile at 10-25, Facultative anaerobes, Catalase +, Oxidase -
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Listeria and food
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tolerate high salt, low pH, multiply at fridge temp, biolfilms on food-processing
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Three lifestyles of L. monocytogenes
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i - intracellular. ii extracular, iii biofilm. change from ii to i by PrfA
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Lifecycle of L. monocytogenes
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1. adhesion to phagosome 2. escape phagosome 3. intracellular multiplication 4. actin-induced motility 5. neighboring cells repeat
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Listeria live in host cells so its...
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sheltered from the immune system
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Virulence factors of L. monocytogenes
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LIL_PA. Listeriolysin O, Listeria Pathogencity Island-1 (LIPI-1), Internalins A and B, Phospholipase C, Actin-Polymerizing protein A
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Listerosis illness
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epidemic or sporadically. Hight hospitalization rate of food illnesses, high mortality rate (20-30%)
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Listeria grow well on
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blood and chocolate agar and Fraser broth
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Specimen growth of listeria
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1 specimen :9 broth. incubate at 4C for days to weeks. Subculture frequently until recovery
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Only 3 species are Beta hemolytic
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L. monocytogenes. L. ivanovii, L. seeligeri (non-pathogenic)
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Primers in Listeria
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hly, fla, ActA, InlA
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|
|
what edition? listeria
|
at first volume 2, then volume 3 firmicutes
|
|
|
What other genera?
|
brochothrix, erysiplothix and Carynanon
|
|
|
Carynanon
|
not clinically important, multicellular - forms trichomes
|
|
|
Domain, phylum, etc. listeria
|
bacteria, firmicutes, bacilli, bacillales, Listeriaceae
|
|
|
Listeria contains what 2 genera?
|
listeria and Brochothrix
|
|
|
How many species of listeria?
|
6 species
|
|
|
L. ivanovii
|
extremely rare human pathogen. Ruminent animals
|
|
|
L. seeligeri
|
rare human pathogen
|
|
|
L. innocua
|
non-pathogenic
|
|
|
L. monocytogenes has ____ serotypes based on what?
|
13 serotypes based on somatic (O) and flagellar (H) anitgens
|
|
|
Serotype __ cause the majority of L. monocytogenes infections
|
4b
|
|
|
What are the 3 lineages based on?
|
three virulence genes
|
|
|
Tell about the three lineages
|
Lineage 1 - most clinical strains. Lineage 2 - some clinical strains. Lineage 3 - mostly animal pathogens
|
|
|
Gram ?, morphology, spores?, capsules?, Acid fast?
|
Gram +, coccoballiary rods, non-encapsulated, non- acid fast
|
|
|
Optimum growth, motile, areobic?, catalase, oxidase
|
optimum temp 30-37C, Motile at 10-25, Facultative anaerobes, Catalase +, Oxidase -
|
|
|
Listeria and food
|
tolerate high salt, low pH, multiply at fridge temp, biolfilms on food-processing
|
|
|
Three lifestyles of L. monocytogenes
|
i - intracellular. ii extracular, iii biofilm. change from ii to i by PrfA
|
|
|
Lifecycle of L. monocytogenes
|
1. adhesion to phagosome 2. escape phagosome 3. intracellular multiplication 4. actin-induced motility 5. neighboring cells repeat
|
|
|
Listeria live in host cells so its...
|
sheltered from the immune system
|
|
|
Virulence factors of L. monocytogenes
|
LIL_PA. Listeriolysin O, Listeria Pathogencity Island-1 (LIPI-1), Internalins A and B, Phospholipase C, Actin-Polymerizing protein A
|
|
|
Listerosis illness
|
epidemic or sporadically. Hight hospitalization rate of food illnesses, high mortality rate (20-30%)
|
|
|
Listeria grow well on
|
blood and chocolate agar and Fraser broth
|
|
|
Specimen growth of listeria
|
1 specimen :9 broth. incubate at 4C for days to weeks. Subculture frequently until recovery
|
|
|
Only 3 species are Beta hemolytic
|
L. monocytogenes. L. ivanovii, L. seeligeri (non-pathogenic)
|
|
|
Primers in Listeria
|
hly, fla, ActA, InlA
|
