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What is the complete aerobic respiration of glucose reaction?
C16H12O2 + 6 O2 --> 6 CO2 + 6 H2O + energy
How do the products of the citric acid cycle contribute to the proton gradient?
NADH and FADH2 generated from the TCA cycle are used by Complex 1 and 2 to pump protons
How is a voltaic cell set us to find reduction potential?
Solution 1 = 1 M X + 1 M X-
Solution 2 = 1 M H+ in equilibrium with 1atm H2 gas
Connect with salt bridge and voltmeter
In a voltaic cell, if test solution takes electrons from the standard solution, the reduction potential is (positive/negative)?
Positive
How do we mathematically calculate free energy of redox reaction?
Standard free energy change = -nF(reduction potential)
n = number of ions passing
F = faraday constant in mol*V
Explain the flow of electrons through the ETC from NADH
NADH --> NADH-Q reductase --> Ub --> Cytochrome c reductase complex --> cytochrome c --> cytochrome c oxidase complex --> O2
Define cytochrome
Protein with a heme cofactor
What is the full name of complex I? What is its net reaction?
NADH-Q Oxidoreductase aka NADH dehydrogenase
NADH + Q + 5 H+ matrix --> NAD+ + QH2 + 4 H+ cytoplasm
Net pumping of 1 proton into matrix
How do electrons flow from NADH to Ubiquinone?
Via complex I
1) NADH + FMN --> NAD+ + FMNH2
2) Through 2Fe-2S and 4Fe-4S clusters and to the Q pool
Explain the proton pumping in NADH-Q oxidoreductase
As e- flow through clusters, 4 H+ are pumped into the intermembrane space from matrix and 2 H+ get taken up from matrix to reduce Q
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