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which two restriction endonucleases at specific recognition sites to produce sticky, cohesive ends?
-EcoRI
-HindIII
which restriction endonuclease cut at specific recognition sites to produce blunt ends?
-HaeIII
which enzyme is used to make complementary DNA (cDNA) from RNA?
- Reverse Transcriptase PCR (RT-PCR)
what does anneal mean?
- to recombine DNA in the double stranded form.
what components does PCR require?
- Taq polymerase
- Mg2+ buffer
- primers forward and reverse
- dNTPs
- DNA template
what do primers do?
- tell polymerase when to work
which enzyme catalyses the formation of RNA form DNA during transcription?
transcriptase
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