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12 Cards in this Set
- Front
- Back
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Masson trichrome
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-differentiate btwn collagen and smooth muscle
-Bouin solution= mordant; Weigert iron hematoxylin= nuclear stain; biebrich scarlet-acid fuschin= acidophilic components; phosophomolybdic /phosophotungstic acid= removes primary stain from collagen; aniline blue= collagen stain -Collagen= blue, smooth muscle & cytoplasm= red; nuclei= black |
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Gomori one step trichrome
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-Bouin→Weigert hem→gomori stain (combination of plasma and connective tissue stain in phoshotungstic acid)
-nuclei= black; collagen & mucus= green; cytoplasm & muscle= red |
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van Gieson
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-van Gieson solution= picric acid + acid fuschin
-stains collagen red and muscle yellow -used as counterstain for the Verhoeff elastic stainm |
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Verhoeff
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-stains elastic fibers
-Verhoeff stain→sodium thiosulfate (removes excess iodine)→ferric chloride (differentiates)→van Gieson -elastic fibers= black; nuclei= blue to black; collagen= red; other tissue elements= yellow |
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Gomori aldehyde fuschin
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-stains elastic fibers
-aldehyde fuschin=primary stain; light green= counterstain -elastic fibers= deep blue to purple; other tissue elements=green |
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Movat pentachrome
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-demonstration of mucin, fibrin, elastic fibers, muscle, & collagen
-nuclei & elastic fibers= black, collagen=yellow; mucin=blue; fibrin= intense red; muscle=red |
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Reticulum
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-ammonical silver impregnation stain that stains for reticular fibers in tissue
-reticulin stains black |
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Phosphotungstic acid hematoxylin (PTAH)
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-demonstrates muscles striations and fibrin
-cross striations and fibrin= blue; nuclei=blue; collagen= red-brown |
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Periodic acid-Methamine silver
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-delineates/stains glomerular basement membranes
-silver impregnation stain w/ periodic acid as the oxidizer -basement membrane= black, background= green |
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Oil red O
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-demonstrates neutral lipids in frozen sections
-Oil red O= primary stain; Harris hem= nuclear stain; propylene glycol=dye solvent/differentiator -Fat= red; nuclei= blue |
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Sudan black B
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-demonstrates neutral lipids.
-most sensitive of lipid dyes -fat= blue-black; nuclei= red |
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Touidine blue
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-demonstrates mast cells which stain metachromatically
-mast cells= deep violet; background= blue |
