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16 Cards in this Set
- Front
- Back
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What are the 5 advantages of latex agglutination?
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1. versatile
2. Rapid 3. no equipment needed 4. Good screening test 5. Long-lived reagents |
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What are the 2 drawbacks of latex agglutination?
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1. Variability btwn manufacturs
2. Not quantitative. |
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What are the 3 advantages of hemagg. inhibition?
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1. Reference methodology
2. Good for IgM assays 3. Easy to read |
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what are the 2 drawbacks to hemagglutination inhibition?
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1. Time consuming
2. Varied sensitivity |
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what are the 2 advantages of EMIT?
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1. Simple to perform
2. No separation step needed |
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what are 2 drawbacks of emit?
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1. Limited to low MW antigens
2. Not as sensitive as other EIAs |
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what are the qualities of ELISA?
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sensitive/specific
-versatile -long-living reagents -variety of detection systems -good for automation -adaptable for screening -qualitative OR quantitative |
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what are the drawbacks of ELISA?
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only cost-effective for batching
-variability of SP carriers -some agents are carcinogenic or expensive |
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what are the advantages to direct or indirect IFA?
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=sensitive
-relatively inexpensive -long-lived reagents -variety of methods -sameday results for DFA |
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what are the drawbacks to IFA?
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-background/nonspcfc fluorscn
-time consuming -interpretation needs expert |
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what is the advantage of RIA?
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sensitive
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WHAT ARE THE DRAWBACKS TO Ria?
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-Disposal of reagents
-Expensive |
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what are the advantages of SPRIA and IRMA?
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-More stable/sensitive/rapid than plain RIA
-measures total amt of antibody -uses less reagent than RIA |
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what are the drawbacks of SPRIA and IRMA?
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same as RIA - disposal/cost
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what are the advantages of complement fixation?
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1. Reference method
2. Relatively sensitive 3. Adaptable to many tests |
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what are the drawbacks to complement fixation?
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-time consuming
-requires many controls -expensive reagents |
