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30 Cards in this Set
- Front
- Back
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Name the curve that represents the distribution of a population.
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Normal distribution curve
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1SD, 2SD, 3SD represents what percentages of the population.
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68%
95% 99.7% |
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State the purpose of calculating the C. V.
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Usefull when comparing the precision of two different methods.
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What pipette requires a blow out?
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Volumetric
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What grade of water is used in most clinical labs?
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I
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Convert 15% to mg/dl
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15,000 mg,dl
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How would you make 1.2 L of a 6.5% (w/v) saline solution?
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Add 78 g and QS up to 1.2L
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What is the percent concentration of 12 g NaCl dissolved in 150 mL of water?
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8%
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What is the percent concentration of 130 mg of HCl + 90 g H20?
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0.14%
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How many grams of NaCl are there in 2.5 L of a 0.4% solution?
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10g
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If 5g of salt are dissolved in 35 g H20, what is the percent concentration of the resulting solution?
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12.5%
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A serum sample is diluted 1/10. How much serum would be present in 20microL of the diluted serum?
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2micro L
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How would you make 50 ml of a 5.8 dilution of urine?
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31.25ml of urine QS up to 50
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How many mL of a 5% KCl solution are needed to make 500ml of a 4% solution?
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V1= 400mLs
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How many mg of a substance would be required to make 200mL of a 25% solution?
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50 x 10>3 mg
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You have on hand a stock standard of 500 mg/dl. What dilution would you use to make a working standard of 25 mg/dL
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1:20 dilution
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Explain the relationship between Concentration and Absorbance as it pertains to spectrophotmetry.
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Direct and Linear
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Explain the relationship between Concentration and %Transmittance.
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Indirect and logarithmic
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Why is the scale for absorbance logarithmic?
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Because light is absorbed logrithmically
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What are four things that can be determined from a standard curve?
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1. Concentration of unknown
2. See if method obeys Beer's Law 3. Determine the extent the method follows Beer's Law 4. Determine sensitivity |
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Where is the highest concentration of calcium found in the body and what is the most common form found in the serum?
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Skeletal-99%
50% of the <.2% found in the serum is in the free form. |
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A calcium level of 13.0 mg/dL would be associated with what sort of symptoms? What are the possible causes for the increase?
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Usually no associated symptoms
Causes include vitamin D overdose, Multiple Myeloma and metastic cancers and tumors. |
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Approximately what percentage of phosphate is found bound to albumin?
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10%
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What stimulates the parathyroid to secrete PTH and what is the overall effect of this action?
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A decrease in Calcium and Magnesium
Increases reabsorption by the kidneys leading to an increase in calcium and magnesium but a decrease in phosphate. |
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Explain the action of Vitamin D.
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Increases absorption of calcium and phosphate in the intestines, whereas PTH stimulates absorption of phosphate in the intestines
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What regulatory hormone is responsible for an increase in phosphate without effecting calcium and magnesium?
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Growth Hormone secreted by the pituitary.
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What regulatory hormone is responsible for an decease in calcium without effecting phosphate and magnesium?
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Calcitonin secreted by the thyroid.
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T4 and aldosterone have what affect on magnesium?
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Decreases
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What effect would hypoabluminemia have on phosphate levels?
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would not be effected because the majority of phosphate found in the serum is either free or complexed. Only 10% is bound to protein
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Poisoning, hypernatremia, uremia and ketoacidosis would all have this effect on the anion gap.
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Would cause an elevated Anion gap due to an increase in positive ions.
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