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35 Cards in this Set
- Front
- Back
A= ebc
plot on abs.(y) vs. conc. (x) |
Beer's Law
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spectrophotometer
has a ____ lamp |
tungsten
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Abs and Transmission
A= |
2-log %T
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scatterplot
relationship between x and y y=mx+b (CE) (m=PE) |
linear regression
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accuracy of 2 means
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T test
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precision of 2 sd
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F test
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percent variability
=sd x 100/ mean smaller= better precision (2-5%) |
CV
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increased probability that result reported is accurate
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QA
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all actions necessary to provide adequate confidence that the lab is providing accurate info
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QC
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date (x) vs. result(y)
shift (4-6) trend(5-7) gradual towards one direction shows loss of precision has westeguard rules |
LJ Chart
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> 2sd
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1 2s
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>3sd
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1 3s
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>2sd in same direction
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2 2s
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> 2sd in opposite direction
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R 4s
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4 samples > 1sd in same direction
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4 1s
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> 10sd on same side
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10x
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1 sd
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68% ci
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2 sd
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95% ci
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3 sd
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99% ci
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<70 mg/dL glc, < 50 = symptoms and intervention needed
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Hypoglycemia
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increased plasma glucose, >500
inbalance of hormones DM |
Hyperglycemia
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child
insulin deficient, ^FPG islet cell anitbodies |
Type 1 DM
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>40 yo
ineffective insulin ^ insulin and FPG due to resistance of tissues to action of insulin obesity, lack of exercise, history, ^ bp |
Type 2 DM
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overprod. of ketoacids
decr. ph > decr. bicarbonate> metabolic acidosis incr. breathing to rid of CO2 coma and ketosis |
DKA
DM1 |
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hyperglycemia>urinary loss of H20, glc, and electolytes w/o replacement
>coma |
Hypersmolar Coma
DM2 |
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pregnancy
due to increase cell resistance to insulin complications- large baby, hypoglycemic baby, defromities and death |
G-DM
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>200
random |
CPG
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>126
8hr fast |
FPG
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>6.5%
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Hgb A1c
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A1c ranges
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N- <5.7
Pre D- 5.7-6.4 D- >6.5% |
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FPG ranges
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N- <100
Pre D- 100-125 D- >126 |
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GOD-POD
glc measurement method reaction |
B-D glucose + O2 (glucose oxidase) > gluconic acid + H2O2
H2O2 + redused dye (peroxidase)> colored oxidized dye + H2O |
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GOD-POD
interferences |
bleach ^
vitamin c- decr |
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Hexokinase method
rxn |
D- glucose + ATP (Mg++) (hexokinase)> G6P + ADP
G6P + NAD (G6PD)> 6-phosphogluconic acid + NADH + H+ |
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Lactic acidosis measurement
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lactate + O2 (lactate oxidase)> pyruvate + H2O2
H2O2 + noncolored chromagen (peroxidase)> colored dye |