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15 Cards in this Set

  • Front
  • Back

Phylogenic

Evolutionary history of an organism - who evolved from who.

Taxonomy

Taking a group of organisms and dividing them into (smaller groups)- reflect relationships between organisms. Also useful for identifying known organisms

Phylogenic Tree

Shows evolutionary history of org. Not including viruses.


3 main groups:


•Archae


•Bacteria


•Eukaryotic


Each branch represents a major evolutionary event.

Staphylococcus aureus

Colonies can be pure white- TSA


Or they can be pale yellow- blood Agar (TSA w/ sheep blood)


•Strains of Staph. aureus (MRSA) Methicillin Resistant S. aureus. Causes toxic shock syndrome and/ or food poisoning enterotoxin and also causes skin infections:boils.

Hierarchy of Classification

Ordering of taxonomic groups from largest to smallest. An inverted triangle w/ domain occupying the top and species occupying the bottom.


This order-->Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species.

Physiology

What is preferred temperature for optimum growth?


-bacteriums relationship to oxygen, is it an aerobe or anaerobe, facultative?


-pH tolerance?


-Most bacteria want pH to be close to neutral, some more acidic or more alkaline.

Growth characteristics of colonies

Pigment


-size


-shape


-texture


-Gram - or Gram +


-Acid fast or non-acid fast

Nutritional requirements

What nutrients can organism metabloize?

Genetic Characteristics

DNA homology studies


-comparing DNA samples from 2 organisms to look at the specific sequence of nucleotides & more similarities we find the more closely related


DNA base composition: ratio of Guanine to Sinine

Phage typing

The identification of bacteria by testing their vulnerability to bacterial viruses.

Phage

Virus that is able to attach to very specific bacteria and infect them. This is how phage infects bacteria and converts it to a virus and reproduces itself. Can determine the type of strain of bacterium by examining it's susceptibility to certain bacteria phages.

Protein analysis

A comparison of proteins samples taken from two or more organisms. More similarities between them, the more closely related.

Microscopic morphology

Shape and arrangement of cells, structural characteristics (like capsules (endospore),etc.)


-staining characteristics Gram + or Gram - & Acid fast or Non-acid fast


-Growth characteristics of media - solid or liquid, pigment of colonies, texture, size and shape.

Bergeys Manual of Determinative Bacteriology (9th edition)

Info on all known and named bacteria. ONLY bacteria in this book.

The International Journal of Systematic Bacteriology

New additions, changes, etc. Are published (species names)