Chromatography

Front 1

Lysis

Back 1

Breaking down of a cell, often by viral, enzymic, or osmotic mechanisms that compromise its integrity.

Done by centrifugation.

Yield a crude extract.

Front 2

Column chromatograph

Back 2

Apply protein mixture to resin or matrix, interacts differently with various protein

Front 3

Purpose of adding buffer

Back 3

Washing away any proteins that do not bind to the matrix

Another is added to dissociate the matrix

Front 4

Elution buffer are chosen

Back 4

so that different proteins dissociate from the matrix

Front 5

Ion exchange chromatograph

Back 5

resin in cation or anion groups

Front 6

Proteins are eluted (Ion exchange chromatograph)

Back 6

depends on the nature of charged amino acid residues

Front 7

Gel-exclusion chromatograph

Back 7

Proteins are separated by size
Resin is composed of hollow beads with pores
Large proteins move around the beads and elute earlier
Small proteins can enter the resin pores and take a longer path through the column

Front 8

Affinity chromatograph

Back 8

Proteins are sorted by the type of ligand, covalently coupled to the column resin