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10 Cards in this Set

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WB Questions

31- What is the purpose of the mouse neutralization test?
To test the presence of botulism in foods.
32- Describe char. which make agar-agar a useful media for bacterial growth?
a- solid @ room temp
b- melts @ boiling temp 100d C
c- once liq-doesn't solidify , cools @ 42deg C
d- flexible, moldable , holds basic framework 2 hold moisture & nutrients
33- Define media: Liquid
Liquid: water based sol/ doesn't solidify @ temp above freezing, free flow container
free flow container is tilted
EX: broths, milks, infusions..can b dispersed, have cloudy appearance
34- Define media: Semisolid
Clot-like consistency, has sml amt of solidifying agent
(agar, gelatin) thickens
but doesn't produce firm sub-
strate

*Determines motility of bact.
35- Name two stains commonly used for their negative staining capabilities:
Nigrosin and India Ink
36- _______ media is used to isolate fungi.
Sabouraud's media
37- Lowenstein-Jensen media is used to isolate:
Mycobacterium
38- ____ agar can be used to identify Salmonella and Shigella.
Mannitol salt
39- Which of the following is NOT a differential stain?
crystal violet
gram, spore, acid-fast, crystal violet & a capsule
40- Explain difference btwn synthetic and non-synthetic media?
Synthetic media composition is chemically defined- has specified exact formula
Non-synthetic media has at least One ingredient not chemically definable