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45 Cards in this Set
- Front
- Back
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Whole Blood |
Measures both the liquid and cellular component |
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Serum |
Liquid part that remains after clotting and centrifugation |
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Plasma |
Liquid part of unclotted blood |
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Accuracy |
W/out error, close to true value |
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Precision |
Closeness of repeated results, expressed as the SD |
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Sensitivity |
Smallest amount of a concentration that can be measured directly |
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Specificity |
method ability to measure only the analyte in question |
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Analyte/solute |
Substance being measured |
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Solvent |
liquid that the solute is located in |
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Which led to the automation we have today ____ & _____? |
Ph meter & colorimeter |
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Advantages of automation |
TAT shorter & small amount of sample needed |
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F= |
(C x 1.8) + 32 |
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C= |
(F - 32) x 5/9 |
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K= |
C + 273 |
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Standard Reference Material (SRM): |
Developed for chemistry, have a verified SD & is called the calibrator
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Grade II Water: |
For reagent/control preparation |
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Grade III Water: |
For cleaning |
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Volumetric flask: |
Used to bring a reagent to final volume |
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Erlenmeyer Flask: |
Used in reagent preparation |
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Graduated cylinder: |
Measure volume of liquid |
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Pipette TC: |
To contain, has certain volume but does not dispense exact volume |
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Pipette TD: |
To deliver, will dispense the stated volume |
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Serological pipette: |
(Blow out pipette) Has # markings to the end of the tip |
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Mohr pipette: |
(Self-draining) Doesn't have number markings on the tip |
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Micropipette: |
(Blow out pipette) Dispenses 1 volume |
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Volumetric Flask: |
(Self-draining) Holds 1 volume of liquids, used for preparing reagent |
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Most critical step: |
Patient ID |
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Order of draw for venipuncture: |
Cultures, blue, yellow/red sst, green, lavender, gray |
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Anticoagulant present: Light blue: Green: Lavender: Gray: |
Light blue: Na Citrate Green: NA/Li/NH3Heparin Lavender: EDTA Gray: K+ oxalate |
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Trough: |
30 minutes before the dose is given, should be low measurement |
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Peak: |
30-60 min. AFTER a 30 min. infusion. Should be higher measurement than trough |
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CSF tubes: Tube 1: Tube 2: Tube 3: |
Tube 1: Chemistry Tube 2: Microbiology Tube 3: Hematology |
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Body fluids: Pericardial: Synovial fluid: Peritoneal: Pleural: |
Pericardial: Heart Synovial fluid: Joint Peritoneal: Abdominal fluid Pleural: fluid from lungs |
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MUST have _______ & ________ |
number value & unit |
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1,000 ug = 1,000,000 ug= |
1 mg 1g |
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Mean: |
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Standard Deviation: |
Indicator of how close values in a set are distributed |
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Variance: |
Defined as the square of the SD |
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Quality assurance |
All actions taken by the lab to ensure that the services provided will satisfy the needs of the patient |
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Quality control |
Alaboratories system for recognizing and minimizing analytical errors |
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Random error: > > * > |
>Due to chance >Possibilities: *Pipetting error, Mixing error, Temperature fluctuation >Repeat testing |
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Systematic error: |
>Data move in 1 direction or another >Rise or fall from established data >Possibilities: *Improper calibration, deterioration, instrument drift >Still out recalibrate, New controls |
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Three types of standards Primary: Standard Reference Materials (SRM): Secondary Standard: |
>Primary: High purity, exact known [ ], expensive and hard to make >Standard Reference Materials (SRM): developed for Chemistry, have a verified SD (standard deviation) >Secondary Standard: Lower purity |
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Water I: |
>Purist >Used when analysis is critical and needs no/few impurities |
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Amniotic Fluid: |
>protect w/ light > Use dark tubes |
