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17 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Types of measurements HbA1c |
2 |
Structural Charge |
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Based on structural differences HbA1c
Examples |
Immunoassays Affinity chromatography |
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Based on charge differences HbA1C
Examples |
HPLC Electrophoresis Ion-exchange chromatography |
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HbA1C
Preferred method |
Affinity chromatography |
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Affinity chromatography Separate based on |
chemical structure glycated from non-glycated based on boric acid |
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Affinity chromatography vs. Interference |
Least affected by interference
But few labs are using |
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Most commonly utilized method in HbA1c |
Immunoassays HPLC |
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How HbA1c used to monitor long term diabetes? |
index of the patient's average blood glucose over a 2-3 month period
Based on RBC lifespan of 100-120 days. |
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HbA1c vs. Fasting |
Fasting is not required |
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NGSP
Full name |
National glycohemoglobin standardization project
Role |
Standardizes Calibration, certification Proficiency testing |
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Method used by NGSP |
Cation-exchange assay method |
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Limitations of NGSP method |
Interference from hemoglobin variants
Examples |
HbAC, D, E, S |
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Most common cause of hemoglobinopathies |
Point mutations |
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Method that can identify abnormal hemoglobin causing HbA1c interferences |
HPLC |
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Limitations of Immunoassays |
Cannot detect abnormal hemoglobin |
Abnormal hemoglobin cannot be recognized antibodies |
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Complication of uncontrolled/long term diabetes |
Renal function is often compromised
Testing method |
MIcroalbumin |
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High levels of microalbumin |
possible nepropathy |
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