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33 Cards in this Set
- Front
- Back
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Increase in number of cells not cell size
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Microbial Growth
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3 temperature ranges organisms grow
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Minimum (10 degrees)
Maximum (60 degrees) Optimim (close to body temp) |
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Cause Food spoilage, grow between 0degrees- and 20-30 degrees Celcius.
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Psychotrophs
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Hypertonic environments, increase salt or sugar, cause Plasmolusis
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Osmotic pressure
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Structural organic molecules, energy source
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Carbon
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In amino acids and proteins
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Nitrogens
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In amino acids, thiamine and biotin
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Sulfur
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In DNA, RNA, ATP and membrands
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Phosphorus
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Inorganice elements required in small amounts
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Trace Elements
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Nutrients prepared for microbial growth
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Culture Medium
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Sterile
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No living Microbes
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Introduction of microbes into medium
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Inoculum
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Microbes growing in/on culture medium
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Culture
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Comples Polysaccharide, used as a solidifying agent for culture media in Petri plates,
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Agar
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Supress unwanted microbes and encourage desired microbes
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Selective media
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Make it wasy to distinguish colonis of different microbes
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Differential media
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Encourages growth of desired microbe
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Enrichment Media
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Rate of incoming medium =
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Rate of removal of medium from vessel (The Chemostat)
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Rate at which medium flows through vessel
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Dilution Rate
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Microbial Contamination
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Sepsis
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The absence of significant contamination
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Asepsis
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Removal of all Microbial Growth
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Sterilization
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Removal of pathogens
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Disinfection
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Removal of pathogens from living tissue
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Antisepsis
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Removal of microbes from a limited area
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Degerming
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Lower microbial counts on eating utensils
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Sanitization
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Kills microbes
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Biocide/Germicide
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Inhibiting not killing microbes
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Bacteriostasis
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Actions of Micobial Control Agents
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Damage protiens
Damage nuclein acids |
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Lowest temperature at which all cellis in a culture are killed in 10 min
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Thermal Death Point
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Minutes to kill 90% of population of cells
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Decimal Reduction Time (DRT)
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Steam under pressure
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Autoclave
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3 types of Disinfectants
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Alcohols
Ethanol Isopropanol |
