Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
14 Cards in this Set
- Front
- Back
|
Bright-field |
Most widely used Specimen darker than surrounding field Used for live and preserved stained specimens |
|
|
Dark-field |
Brightly illuminated specimens surrounded dark field Used for live unstained specimens Dark background, can't see internal structures |
|
|
Phase contrast |
Transforms subtle changes in light waves passing through the specimen into differences in light intensity |
|
|
Fluorescent microscope |
Modified microscope with an ultraviolet radiation source filter |
|
|
Wet mounts |
Allow examinations of characteristics of live cells: size, motility, shape, and arrangements. |
|
|
Fixed mounts |
Permit visualization of cells or cell parts. |
|
|
Basic dyes |
Cationic, positively charged chromophere |
|
|
Positive staining |
Surfaces of microbes are negatively charged and attract basic dyes |
|
|
Acidic dyes |
Anionic, negatively charged chromophere |
|
|
Negative staining |
Microbe repels dye, the dye stains the background |
|
|
Simple stains |
One dye is used; reveals shape, size, arrangement |
|
|
Differential stains |
Use a primary stain and a counter stain to distinguish cell types or parts (Ex. Gram stain, acid-fast stain, and endospore stain) |
|
|
Structural stains |
Reveal certain cell parts not revealed by conventional methods: capsule and flagellar stains |
|
|
Isolation technique |
Streak plate technique |
