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10 Cards in this Set
- Front
- Back
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Which antiobotic inhibit the procariotic RNA Polimerase |
Rifampicina and Actinomicin D |
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Which structure is required to iniciate the translation for the Expression of a Procaryotic Protein Coding gene |
Shine Dalgardo Sequence (Protein syntesis begins at an AUG codon) |
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Which sub unit ribosomal is inactivated by the Shiga Toxin (Shigella disenteriae) in the eucariote ribosome? |
28S rRNA in the 60 subunit |
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PROMOTER |
Is a region of DNA that initiates transcription of a paticular gene.
A protein factor called sigma is required for the initiation of trnascription at a promoter. Termination of transcription sometimes requires a protein called rho factor. |
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Comparison of RNA Polymerase |
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Prokaryotic Transcriptase Unit |
This is going to obtain: 1) Promoter element 2) Coding region The bacterial promoter contains two "consensus" sequences, called the Pribnow box (or TATA box) |
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Procariotic mRNA |
Polycistronic (contains more than one genes sequences in one particular mRNA) No introns Tc and Tl are couple. The ribosome is the only unusued unic structure in the cell that is actually going to read from 5' to 3'. |
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Eukaryotic mRNA |
Monocistronic Maduration (capping, apicing, poly-adrnylation). Tc and Tl ar not couple. |
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tRNA FUNCTION |
Pick up amino acid Recognize codon on mRNA. |
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Initiation of transcription in Prokaryotic and Eukariotic cells |
Procaryotic= promoter (-10) TATAAT box and (-35) sequece. Eukaryptic= promoter (-25) TATA box and (-70) CAAT. Transcription factor (TFIID) bind promoter. |
