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30 Cards in this Set
- Front
- Back
bacteriophages
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virusus that infect bacteriA
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plasmid
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a small circular dna molucule that may be present and replicate inside a bacterial cell
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transformtion
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the incorpatration of genetic material into a cell
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palindromic sequences
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base sequence of one strand of dna reads the same as compliment when both are in in 5-3 direction
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dna ligase
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an enzyme that covantley links two dna fragments to form a stable combinant dna molecule
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cosmid cloning vectors
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combination vectors with features from both bacteriophages and plasmids
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genonme
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total dna in a cell
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chromosome library
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containing all the dna fragments in that specific chromosome
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colony
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a clone og genetically identical cells orginating from a single cell
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genetic probe
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a segment of dna that is homologous to part of the sequence
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hybridize
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attach by base pairing
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introns
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eukaryoatic genens that do not code for protein
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cDNA
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complementray to messenger RNA
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reveerse transcript
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synthezies single stranded cdna
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poymerse chain reaction
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reaction the amplifies DNA millions of times in few hrs
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gel electrophoreis
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carry charged groups that cause them to migrate in an electriacle field
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Southern blot
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methos of decting dna fragments
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WEstern blot
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blot consisting of proteins or plypeptides previsolousy sperated by gel electrophoresis
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Northen blot
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When Rna molecules are transferred to membrane
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RFLP's
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used to determine how cloesly related differnt memebers of a population are
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Genomics
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study of dna sequencing
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Ests
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help identify protein coding genes
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gene targeting
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a prcedue when you knock out a single gene in a organism
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dna microarrays
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provide a way to study patterns of gene expression
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pharmacogenetics
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customizing drugs to match a person's genetic makeup
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proteomics
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study of persons genome and cells
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gene therapy
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the use of specific dna to treat diease by correcting the genetic problem
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STRS
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moleculars markers that are short sequnces of reptive dna
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retrovirus
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make dna copies of themsleves by reverse transcription
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rubisco
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key carbon fixing enzyme in phtotsyntheiss
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