Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
8 Cards in this Set
- Front
- Back
|
What is an Indicator Organism? |
Used to measure fecal contamination to assess water quality. E.Coli is commonly used |
|
|
What are four properties of an indicator organism? |
1) easy to detect 2) only natural environment of the microbe is associated with feces and should always be present 3) should not grow outside of its natural environment 4)should survive longer than most viable pathogen but not too long as historical events are detected |
|
|
What are Coliforms? |
Closest to fulfilling the four properties as an indicator organism and measures contamination in the water. Subset of the Enterics.. non-sporeforming, facultatively anaerobe,gram negative and ferments lactose + acid + gas |
|
|
What is Enterics? |
A large group of gram negative, non sporulating, short rods.. the Enterobacteriacoea species |
|
|
What is a fecal coliform? |
type of coliform exclusively associated with fecal matters of warm blooded animals. E coli is a good indicator for fecal pollution because they do not last long enough outside the host and can be differentiated from the others by selective media and 44.5* incubation |
|
|
How to quantify Bacteria in Food and Water? |
Use either the Coliert test with MPN quantification or serial dilution with plate count |
|
|
Coliert Test |
Predicts the MPN of coliform and fecal coliform present in water samples. |
|
|
How are positives determined in the coliert test? |
Using ONPG and MUG which turns yellow or fluorescent |
