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23 Cards in this Set

  • Front
  • Back

Sterilization

The destruction of all life forms including endospores; (strictly speaking not possible because prions are tough to destroy.)

Disinfection

Destruction of pathogenic organisms on inanimate objects; may not destroy endospores.

Antisepsis

The destruction of pathogenic organisms on living organisms.

Degerming

Physical removal of microorganisms fro


Surfaces; e.g. Washing hands and taking a bath

Sanitation

Reduction of microorganisms to a level deemed safe by public health guidelines as in restaurants.

Sepsis

Bacterial contamination

Bacteremia

Presence of bacteria in blood

Septicemia

Presence of actively reproducing bacteria in blood.

Suffixes


-cide


-stat or stasis

-cide: to kill; biocide, germicide, fungicide, etc.


-stat or stasis: inhibit the growth of; fungistasis, bacteriostatic

Methods controlling microorganisms

Physical means


Chemical means


Mechanical means

Efficiency depends on: (5)

-# of microbes


-microbe characteristics


-environment


-concentration and mode of action


- organic matter

Physical methods

-heat


-radiation


-filtration

Heat types (2)

- moist heat


-dry heat

Moist heat

-boiling


-pasteurization


-steam under pressure

Boiling

100 degrees Celsius for 10-30 minutes

Pasteurization

Reduces spoilage caused by microbes and kills pathogens.

Steam under pressure

Autoclaving 121 degrees Celsius 15 psi for 15 minutes (destroys spores)

Dry heat (3)

-flaming (inoculating)


-hot air


-incineration

Hot air

Effective at 170 degrees Celsius for 2 hours

Incineration

Destroys microbes to ashes or gas


-Bunsen burner 1870 degrees Celsius


-furnace 800-6500 degrees Celsius

Radiation

-ionizing: X-rays, gamma rays, electron beams


-nonionizing: UV and microwaves

Filtration

Removes microbes and spores from liquids and air

Classes of chemical agents (10)

-phenols


-biguanides


-halogens


-alcohols


-heavy metals


-surfactants


Aldehydes


-peroxygens


-gases


-dyes, acids, and alkaline