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41 Cards in this Set

  • Front
  • Back

What are the advantages of light microscopes?

-Cheap


-Easy to use


-Portable


-Can study whole living specimens

What is the magnification of optical microscopes?

X1500

What is the definition of resolution?

The ability of a microscope to produce an image that shows fine detail clearly


(The clarity of an image)

What is the wavelength range of visible light?

400-700 nm

What are laser scanning microscopes also known as?

Confocal

What are laser scanning microscopes used for?

-Biological research


-Medicine

How do laser scanning microscopes work?

Scan an object point by point and assemble information into an image on a computer

What microscope has depth selectivity?

Laser scanning

Are the resolution and contrast of a laser scanning microscope high or low?

High

How do transmission electron microscopes work?

-Specimen is chemically fixed and dehydrated


-Beams of electrons pass through specimen


-Forms a 2D black and white image

What is the magnification of transmission electron microscopes?

X2 million

How do scanning electron microscopes work?

-Specimen is placed in a vacuum and coated in metal


-Electrons bounce off surface of specimen and are focused on a screen


-Produces a 3D image, black and white but can be given false colour

What is the magnification of scanning electron microscopes?

Up to X200000

What are the disadvantages of electron microscopes?

-Large/expensive


-Need training to use one


-Specimens have to be dead

Name a substance that leaves the nucleus through nuclear pores

RNA

Name a substance that enters the nucleus through nuclear pores

Steroid hormones

What is the name of the double membrane surrounding the nucleus?

Nuclear envelope

What is contained in the nucleolus?

RNA

What are made in the nucleolus?

Ribosomes

What is the name of the genetic material consisting of DNA wound around histone proteins?

Chromatin

Name the four main functions of the nucleus

-Control centre


-Stores genome


-Transmits genetic information


-Provides instructions for protein synthesis

Describe the structure of the RER

-System of membranes containing fluid-filled cavities that are continuous with the nuclear membrane


-Coated in ribosomes

What is the name of the fluid-filled cavities in the RER and SER?

Cisternae

Describe the structure of the SER

-System of membranes containing fluid-filled cavities that are continuous with the nuclear membrane


-No ribosomes on the surface

What are the functions of the RER?

-Intracellular transport system, cisternae form channels


-Large surface area for ribosomes

What are the functions of the SER?

-Contains enzymes that catalyse lipid metabolism reactions (eg synthesis of cholesterol)


-Involved with absorption/transport/synthesis of lipids

Describe the structure of the Golgi apparatus

Stack of membrane-bound flattened sacs

What are the functions of the Golgi apparatus?

-Modifies proteins (folds into their 3D shape, adds sugar molecules to form glycoproteins)

What do vesicles do at the Golgi apparatus?

Have proteins packaged in them, are pinched off, then stored in cell or move to the plasma membrane to be exported

Describe the structure of mitochondria

-2-5um


-Spherical/rod-shaped/branched


-Surrounded by two membranes, inner membrane is folded into cristae


-Inner part is a fluid-filled matrix

What are the functions of mitochondria?

-Site of ATP production


-Self replicating (more can be made if cell's energy needs to increase)


-Abundant in cells where a lot of metabolic activity takes place

Describe the structure of chloroplasts

-Large organelles (4-10um)


-Surrounded by a double membrane


-Inner membrane is continuous with stacks of flattened membrane sacs called thylakoids (contain chlorophyll)


-Piles of thylakoids=grana


-Contains loops of DNA and starch grains

What are the functions of chloroplasts?

-Site of photosynthesis


-Light energy trapped and used to make ATP in grana


-Abundant in leaf cells (palisade mesophyll layer)

Describe the structure of vacuoles

Surrounded by a membrane called the tonoplast, contains fluid

What are the functions of vacuoles?

Filled with water, maintains cell stability and makes cell turgid

Describe the structure of lysosomes

-Small bags formed from Golgi apparatus


-Contain powerful hydrolytic enzymes


-Abundant in phagocytic cells

What do lysosomes do in phagocytic cells?

Ingest and digest invading pathogens

What are the functions of lysosomes?

-Keep the hydrolytic enzymes separate from the rest of the cell


-Can engulf old organelles/foreign matter and digest them

What is the only human cell to have an undulipodium?

Spermatozoon

What are cilia/undulipodia?

-Protrusions from the cell that are surrounded by the cell surface membrane


-Contain microtubules


-Formed from centrioles

What are the functions of cilia/undulipodia?

-Cilia in airway move mucus


-Nearly all cells have one cilium that acts as an antenna


-Undulipodium allows spermatozoon to move