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31 Cards in this Set

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eukaryotic cell cycle ⭐️pic

-G1➡️S➡️G2➡️M


-G1/S/G2 = interphase


-no active division


-chromosomes are replicated in preparation for mitosis


-chromosomes appear as extended filaments and cannot be seen with a light microscope


1. G1 phase: gap 1, growth, prepares for DNA replication


2. S phase: DNA is replicated, chromosomes = two sister chromatids


3. G2 phase: gap 2, prepares for cell division


4. M phase: final cell cycle phase (1/2-2 hours)


-G0 = resting phase, cells that are eventually going to die

mitosis

-cell division where single cells reproduce themselves


-daughter cells identical to each other and the parent cell


-all cells = diploid (2 copies of each chromosome)


-four phases:


1. prophase


2. metaphase


3. anaphase


4. telophase


-IPMAT: interphase prophase metaphase anaphase telophase

mitosis prophase (pic)

-less than 1/2 of mitosis is spent in prophase


1. centrioles migrate to form poles


-poles = axis for chromosomal segregation


-chromatin fibers begin to condense ➡️ "hallmark feature of prophase"


-threadlike chromosome become visible


-double structure with 2 chromatids held together by centromere


2. spindle fibers begin to form


-pull chromatids apart to opposite poles

mitosis prometaphase (pic)

-nuclear membrane disappears


-spindle fibers make contact with chromosomes


-chromosome move to the equatorial plane "metaphase plate", led by centromere

mitosis metaphase (pic)

-chromosomes are fully condensed


-alignment on the metaphase plate


-centromere is along the plate with chromosome arms extended toward the poles


-sister chromatids remain intact but are ready for migration to opposite poles in anaphase


-occurs via the spindle fibers attached to the kinetochore

kinetochore (pic)

-protein structure associated with the centromere

anaphase (pic)

-shortest phase of mitosis


-sister chromatids disjoin and migrate to opposite ends of the cell


-spindle fibers pull the chromatids apart at the centromere


-each chromatid is now called "daughter chromosome"


-at end of anaphase each pole has identical set of chromosomes (2n)

telophase (pic)

-final stage of mitosis


-complete set of chromosomes at each end


-cytokinesis: division of cytoplasm into two cells (plants = cell wall develops where metaphase plate was, animals = plasma membrane is constricted/pinched into 2 cells)


-chromosomes uncoil into chromatin


-PM reforms


-2 identical daughter cells with identical copies of the genetic material

mitosis vs meiosis (pic)

-mitosis: daughter cells with same amount of genetic material as paternal - one duplication, one division (diploid ➡️ diploid)


-meiosis: daughter cells with half the genetic material as paternal - one duplication, two divisions (diploid ➡️ haploid)

meiosis overview

-two successive nuclear divisions (meiosis I + meiosis II)


-produces 4 cells: each with only one member of each pair of pre-meiotic cell chromosomes


-"meioum" = "to reduce" (Greek)


-homologous chromosomes pair during meiosis to allow for reduction all division - "key feature"


-interphase is prior to meiosis just like mitosis (DNA/chromosomes replicate)


-stages of meiosis


1. meiosis I: first meiotic division, reductional division


-prophase I, metaphase I, anaphase I, telophase I


2. meiosis II: second meiotic division, equational division


-prophase II, metaphase II, anaphase II, telophase II

meiosis I prophase I (pics)

-several days


1. leptotene


2. zygotene


3. pachytene


4. diplotene


5. diakinesis


-main feature of meiosis


-important to understanding how meiosis ⬆️genetic diversity

meiosis I prophase I leptotene

-chromosomes long/threadlike structures


-DNA was duplicated during prior interphase


-chromosomes condense and become visible

meiosis I prophase I zygotene (FIND A PIC)

-homologous chromosomes pair (synapsis)


-synapsis is facilitated by the synaptomenal complex: ribbon that forms between the paired chromosomes


-paired homologous = "bivalents"


-four chromosomes = "tetrad" - where can see sister chromatids

meiosis I prophase I pachytene (pic)

-synapsis is complete (all paired)


-sister chromatids are visible


-each homologous pair has 4 chromatids


-crossing over occurs (not obvious until next phase): physical exchange of genetic material between chromatids of homologous chromosomes (NOT SISTER CHROMATIDS)


-chromosomes become a mosaic of the maternal and paternal homologs


-⬆️genetic diversity


-chiasma: point of crossing over


-genetic recombination

meiosis I prophase I diplotene

-chromosomes get shorter/thicker


-homologous chromosomes begin to separate


-each chromosome pair had at least one or more chiasma


-chiasma = result of breakage and rejoining between nonsister chromatids = point of crossover


-crossover become visible ⭐️⭐️⭐️

meiosis I prophase I diakinesis

-fully condensed chromosomes


-homologous chromosomes repel each other, held together by chiasma


-nuclear membrane breaks down


-spindle fiber formation is initiated

meiosis I prophase I overview

(pic)


-leptotene: condense + visible


-zygotene: synapsis


-pachytene: crossover


-diplotene: chiasma crossover = visible


-diakinesis: fully condensed, homologs repelling each other, only held together at chiasma

meiosis I metaphase I

-alignment


-tetrads move and align on equatorial plane


-centromeres of bivalents homologs are positioned toward opposite poles

meiosis I anaphase I

-homologous chromosomes are drawn to opposite poles


-homologs disjoin (disjunction occurs)


-centromeres do not divide so each chromosome is still 2 sister chromatids


-random separation of chromosomes


-Mendelian independent assortment

meiosis I telophase I

-chromosomes are at the poles


-haploid set at each pole


-one homolog from each bivalent at each pole


-cytoplasm divides

interkinesis

-period between meiosis I and II


-nuclear membrane forms around each set


-chromosomes uncoil/decondense and each one still consists of two sister chromatids


-(formation of two daughter cells may or may not occur as in mitosis)

meiosis I overview (2 pics)

-metaphase I: some are mosaics, result of crossing over

meiosis II overview

-resembles normal mitosis (separation of sister chromatids)


-only one member of a chromosome pair in the nucleus


-the chromosome number remains the same in each cell before and after the second division (equational division)


-no DNA replication occurs before meiosis II

meiosis II phases (pic)

1. prophase II: chromosomes condense, nuclear envelope breaks down, begin moving toward equatorial plate, spindle fibers reform


2. metaphase II: chromosomes align on equatorial plate


3. anaphase II: centromeres (kinetochores) separate, chromatids disjoin (move to opposite poles), each chromatids is a distinct chromosome


4. telophase II: chromosomes reach the poles, nuclear membranes form around each set of chromosomes, cytokinesis occurs (cytoplasm divides)


➡️four daughter cells


➡️each contains a single (haploid) set of chromosomes that become gametes

animal oogenesis (pic)

-formation of mature egg cells (oocytes = meiocytes)


-in a female 3 of the haploid cells made in meiosis contain little cytoplasm and degrade


-one cell contains majority of cytoplasm (majority of cytoplasm goes into that cell at each division) and becomes egg (ovum)


-polar body at each step, usually just dissolve, don't divide and move forward


-females are born with all of the eggs (oocytes) they will ever possess


-first division begins in the embryonic ovary: stops in prophase I (suspended in diplotene), resumes prior to ovulation


-second division: completed after fertilization


-one diploid germ cell ➡️ one product

animal spermatogenesis (pic)

-spermatogenesis: formation of mature sperm cells


-all four haploid cells produced during meiosis become functional sperm cells


-among the smallest cells in an organisms


-consist of a head and tail: head had enzymes to break down the egg's plasma membrane so sperm can enter, tail has mitochondria to provide energy to move sperm


-fertilization: occurs with the union of egg and sperm

plant spores

-spores: haploid cells produced in meiosis, divides mitotically to produce a gametophyte


-gametophyte: fully developed multicellular haploid organism

megasporogenesis (pic)

-megasporogenesis: development of embryo sac in plants


-megaspores: 4 haploid cells produced in meiosis, only one megaphone is carried forward/three degrade


-⭐️in both male and female flowers?


-mature female gametophyte (embryo sac): nucleus of the surviving megaspores divides mitotically 3 times but does not cytokinese (8 nuclei at end)


-3 antipodal cells, 2 polar nuclei, 2 synergids, egg

plant microsporogenesis

-microsporogenesis: pollen grain development


-4 haploid cells produced in meiosis are called micro spores (each can become a functional pollen grain)


-a microspore nucleus will divide mitotically to form 2 nuclei within the original spore wall


1. tube nucleus: makes pollen tube


2. generative nucleus: divides again producing 2 sperm nuclei


➡️ entire structure (TN + GN) = pollen grain

plant fertilization

-embryo sac with a pollen grain


-one sperm nucleus unites with the egg to produce zygote (embryo)


-the other sperm nucleus unites with the 2 polar nuclei and becomes the endosperm


➡️ double fertilization


-embryo = diploid (egg n + sperm n = 2n)


-endosperm = triploid (polar nucleus n + polar nucleus n + sperm n = 3n)

plant gamete formation/fertilization cycle overview (pic)

-megasporogenesis


-microsporogenesis