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30 Cards in this Set
- Front
- Back
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what are the four major functions of the cell membrane
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define boundaries
site of some cell function control in and out role in communication |
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what does the "site of some cell function" mean?
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enzymes proteins pigments and lipids
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some proteins float freely in the PM others are anchored in the _______-
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cytoskeleton
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what does patching and capping show
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experiment with anti rabbit shoes patching and capping showing that proteins could move
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what are the two classes of membrane prtoeins
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integral and peripheral
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membrane lipids dissolve well in ____ solvents
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organic
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how can we analyze membrane lipids
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TLC and LLC
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what does LCC stand for
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liquid column chromotagraphy
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describe how we can analyze membrane proteins
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solubize membranes with detergent
extract proteins resolve proteins on gel stain/blot for proteins SDSPAGE allow - proteins to move toward the anode |
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what does resolve mean
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separate
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what does SDS PAGE stand for
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sodium dodecyl sulfate polyacrylamide gel electrophoresis
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what si the SDSPAGE
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the gel surface used to pick up the proteins for gel electrophoresis
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what proteins move fast and slower
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little moves faster
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rate of protein movement is _____ proportional to molecular weight
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inversely
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in western blot proteins move toward the _____
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anode
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is the anode positive or negative
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+ the proteins are negative
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how do you complete the western blot from the sDS PAGE
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immerse membrane in soln containig Abs to select prot
add chemiluminescent reagent place film use film to determin desitometry |
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what does reticulum mean?
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network
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the ER deivides the cell into ______ and ______ spaces
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cytoplasmic
cisternae |
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what is included in the ER
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golgi lysosomal and perinuclear spaces
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visually how could you distinguish SER from RER
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tubes vs flattened sacs
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what does the ser do
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detox via hydroxylation of intermediates
carb metabolism lipid synthesis increase leydig cell or granulosa cells steroid synthesis |
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what does the ser synthesize
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steroids
lipids |
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how does the ser carry out carb metabolism
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glycogenolysis
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how does the ser carry out detox
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hydroxylation of intermediates
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the RER is a _____ _____ protein
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membrane associated
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where would you see more SER
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leydig cells or granulosa cells
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where would you see increased RER
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pancreatic
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what is the other membrane associated protein
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golgi complex
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what comes fromt eh trans face of the golgi complex
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secretory vesicles
lysosomal vesicles emmbrane bound vesicles peroxisomal vesicles |
