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14 Cards in this Set

  • Front
  • Back
when is the specific activity of a particular enzymatic activity of particular importance?
when proteins are being purified or different sample are being compared.
what do the most utilized methods to assay total protein rely on
the reduction of copper in the presence of a chromogenic reagent
_____ is the most widely used method for accurate determination of protein concentration
lowry's method of protein estimation
lowry's method is a combination of what two reactions?
biuret and folin ciocalteau
what is the first step of the reaction

and in the second step?

what does this reaction produce and what does it predominantly depend on
protein binds to copper in alkaline medium and produces cu++

cu++ catalyses oxidation of aromatic amino acid by reducing phosphomolybdotungstate to heteropolymolybdanum blue

strong blue color

tyrosine and trytophan content of protein to a lesser extent cysteine
why do we add BCA reagent after lysing cells?
to provide copper ion which is going to adhere and turn blue
copper absorbs light at __nm
562
transmittance is how much is ____

the spectrophotometer reads out ____

how do you find OD
emitted

absorbance

1/log(A)
_____ are going to absorb light from spectrophotometer
buffer reacted with protein
what are the units for conc of protein
micro grams/mL
standard is a known quantity used as a control

how can we use this
based of known we can predict unknown samples
the higher the OD the ____ the proteins
higher
what was the blank 50ml buffer used for
to calibrate the spectrophotometer
the concentration of total protein from the cells contain what kind of proteins
membrane nuclear and cytosolic proteins