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15 Cards in this Set

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cultured cells endycytose solutes and particles from their extracellular medium at high rates

know two example of such cells
fibroblasts and macrophages
what is the general endocytic pathway
early endosome late endosome lysosomes
what is pinocytosis or cell drinking?
the mechanism by which a cell incorporates fluids or particle less than 5 microns, from the external environment inside the cell through INVAGINATION OF THE CELL MEMBRANE
the process of pinocytosis requires energy from _____
adenosine triphosphate
pinocytosis is used primarly for absorption of ____, and it generates _____
extracellular fluids ECF and it generates very small vesicles
what is the primary different of pinocytosis and phagocytosis ?
pinocytosis is non specific in the substances that it transports. the cell takes in surrounding fluids, including all solutes present

phagocytosis is specific in the substances it transports
phagocytosis actually engulfs _____, which are later broken down by _____ and finally _____
whole particles
enzymes such as lysosomes
absorbed into the cells
pinocytosis is when a cell engulfs already _____ or ____ food
dissolved or broken down
what is horseradish peroxidase?
an enzyme used to label antigens and their antibodies

belongs to peroxidase groups of enzymes extracted from horse radish plant
what is a peroxidase?
any group of enzymes that catalyze the oxidation of a compound by a peroxide
the objective of the lab was to label _____ with a soluble marker ___ to observe ___
labeling the endocytic compartment with soluble marker (HRP) to observe the rate of pinocytosis
what is the purpose of enzyme assay?
to determine the internalized marker HRP to observe the rate of pinocytosis
A1 fluid phase marker HRP = uptake in ____ cells
fibroblast cells
name the seven materials we used
macrophage cell monolayers in plate

alpha minimal essential medium

bovine serum albumin (BSA)

phosphate buffered saline PBS

cell scrapper

triton X
what is PBS used for
help to maintain a constant pH. The osmolarity and ion concentrations of the solution usually match those of the human body (isotonic).